Acute effects of gentamicin on thick ascending limb function in the rat

David T. Kidwell, J. Wade McKeown, Jay S. Grider, Glenn B. McCombs, Cobern E. Ott, Brian A. Jackson

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

It is well established that the aminoglycoside antibiotics can adversely affect proximal tubule function. Predominantly indirect evidence suggests that aminoglycosides may also affect function of more distal nephron segments. The present study utilized whole kidney clearance, in vivo micropuncture and in vitro microperfusion to directly determine whether acute gentamicin treatment affects sodium chloride transport in the thick ascending limb of the loop of Henle. Gentamicin (25 mg/kg) significantly increased urine flow, as well as sodium, potassium and chloride excretion within 15 min of intravenous injection. Glomerular filtration rate and proximal tubule fluid reabsorption were not altered by acute gentamicin treatment. In contrast, both fractional and absolute loop chloride transport was significantly decreased. In the in vitro microperfused medullary thick ascending limb, luminal but not basolateral administration of gentamicin (1 mM) significantly decreased chloride reabsorption when compared to time controls. These data suggest that the increased urine and electrolyte excretion associated with acute gentamicin treatment is, at least in part, a consequence of decreased transport in the thick ascending limb of Henle's loop.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalEuropean Journal of Pharmacology: Environmental Toxicology and
Volume270
Issue number1
DOIs
StatePublished - Jan 3 1994

Keywords

  • Aminoglycoside
  • Loop of Henle
  • Microperfusion, in vitro
  • Micropuncture, in vivo
  • Tubular transport

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology
  • Pollution

Fingerprint

Dive into the research topics of 'Acute effects of gentamicin on thick ascending limb function in the rat'. Together they form a unique fingerprint.

Cite this