Adaptation mechanism and tolerance of Rhodopseudomonas palustris PSB-S under pyrazosulfuron-ethyl stress 06 Biological Sciences 0601 Biochemistry and Cell Biology

Xiang Wen Luo, De Yang Zhang, Teng Hui Zhu, Xu Guo Zhou, Jing Peng, Song Bai Zhang, Yong Liu

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Background: Pyrazosulfuron-ethyl is a long lasting herbicide in the agro-ecosystem and its residue is toxic to crops and other non-target organisms. A better understanding of molecular basis in pyrazosulfuron-ethyl tolerant organisms will shed light on the adaptive mechanisms to this herbicide. Results: Pyrazosulfuron-ethyl inhibited biomass production in Rhodopseudomonas palustris PSB-S, altered cell morphology, suppressed flagella formation, and reduced pigment biosynthesis through significant suppression of carotenoids biosynthesis. A total of 1127 protein spots were detected in the two-dimensional gel electrophoresis. Among them, 72 spots representing 56 different proteins were found to be differently expressed using MALDI-TOF/TOF-MS, including 26 up- and 30 down-regulated proteins in the pyrazosulfuron-ethyl-treated PSB-S cells. The up-regulated proteins were involved predominantly in oxidative stress or energy generation pathways, while most of the down-regulated proteins were involved in the biomass biosynthesis pathway. The protein expression profiles suggested that the elongation factor G, cell division protein FtsZ, and proteins associated with the ABC transporters were crucial for R. palustris PSB-S tolerance against pyrazosulfuron-ethyl. Conclusion: Up-regulated proteins, including elongation factor G, cell division FtsZ, ATP synthase, and superoxide dismutase, and down-regulated proteins, including ALS III and ABC transporters, as well as some unknown proteins might play roles in R. palustris PSB-S adaptation to pyrazosulfuron-ethyl induced stresses. Functional validations of these candidate proteins should help to develope transgenic crops resistant to pyrazosulfuron-ethyl.

Original languageEnglish
Article number207
JournalBMC Microbiology
Volume18
Issue number1
DOIs
StatePublished - Dec 7 2018

Bibliographical note

Funding Information:
This work was financially supported by the National Key R&D Program of China (2017YFD0800702), the National Natural Science Foundation of China (grants 3140110978), the Agriculture Research System of China (CARS-23-D-02), and Hunan Talent Project (2016RS2019). The funders had no contribution on study design, data analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2018 The Author(s).

Keywords

  • Adaption mechanism
  • Cytology
  • Proteomic
  • Pyrazosulfuron-ethyl
  • Rhodopseudomonas palustris PSB-S

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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