Abstract
Increased permeability and reduced cerebral endothelial cell (CEC) viability induced by oxidative stress are the hallmarks of the blood-brain barrier disruption. In our experiments hydrogen peroxide (H2O 2, 0.5 mM) induced a continuous decrease of the transendothelial electrical resistance (TEER) and resulted in intercellular gap formations in cultured rat CECs. Adrenomedullin (AM) increased TEER, enhanced peripheral localization of F-actin bands and attenuated the increased permeability induced by H2O2. Furthermore, AM treatment preserved mitochondrial membrane potential, attenuated cytochrome c release, and consequently improved CEC viability in H2O2 treated cultures. These results suggest that AM treatment protects CECs against oxidative injury.
Original language | English |
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Pages (from-to) | 27-34 |
Number of pages | 8 |
Journal | Regulatory Peptides |
Volume | 130 |
Issue number | 1-2 |
DOIs | |
State | Published - Aug 15 2005 |
Bibliographical note
Funding Information:This work was supported by grants from Ajinomoto Co., Ltd (Japan) for Y. Ueta, Grant-in-Aid for the promotion of Occupational Health for the Post Graduate Student of the University of Occupational and Environmental Health for L. Chen and NIH (HL-30260, HL-46558, HL-50587, DK-62372) and AHA Bugher Foundation Award (0270114N) for D. W. Busija.
Keywords
- Adrenomedullin
- Hydrogen peroxide
- Permeability
- Rat cerebral endothelial cell
- Viability
ASJC Scopus subject areas
- Biochemistry
- Physiology
- Endocrinology
- Clinical Biochemistry
- Cellular and Molecular Neuroscience