Adrenomedullin protects rat cerebral endothelial cells from oxidant damage in vitro

Lei Chen, Bela Kis, David W. Busija, Hiroshi Yamashita, Yoichi Ueta

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Increased permeability and reduced cerebral endothelial cell (CEC) viability induced by oxidative stress are the hallmarks of the blood-brain barrier disruption. In our experiments hydrogen peroxide (H2O 2, 0.5 mM) induced a continuous decrease of the transendothelial electrical resistance (TEER) and resulted in intercellular gap formations in cultured rat CECs. Adrenomedullin (AM) increased TEER, enhanced peripheral localization of F-actin bands and attenuated the increased permeability induced by H2O2. Furthermore, AM treatment preserved mitochondrial membrane potential, attenuated cytochrome c release, and consequently improved CEC viability in H2O2 treated cultures. These results suggest that AM treatment protects CECs against oxidative injury.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalRegulatory Peptides
Volume130
Issue number1-2
DOIs
StatePublished - Aug 15 2005

Bibliographical note

Funding Information:
This work was supported by grants from Ajinomoto Co., Ltd (Japan) for Y. Ueta, Grant-in-Aid for the promotion of Occupational Health for the Post Graduate Student of the University of Occupational and Environmental Health for L. Chen and NIH (HL-30260, HL-46558, HL-50587, DK-62372) and AHA Bugher Foundation Award (0270114N) for D. W. Busija.

Keywords

  • Adrenomedullin
  • Hydrogen peroxide
  • Permeability
  • Rat cerebral endothelial cell
  • Viability

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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