Abstract
Mouse digit amputation provides a useful model of bone growth after injury, in that the injury promotes intramembranous bone formation in an adult animal. The digit tip is composed of skin, nerves, blood vessels, bones, and tendons, all of which regenerate after digit tip amputation, making it a powerful model for multi-tissue regeneration. Bone integrity relies upon a balanced remodeling between bone resorption and formation, which, when disrupted, results in changes to bone architecture and biomechanics, particularly during aging. In this study, we used recently developed techniques to evaluate bone patterning differences between young and aged regenerated bone. This analysis suggests that aged mice have altered trabecular spacing and patterning and increased mineral density of the regenerated bone. To further characterize the biomechanics of regenerated bone, we measured elasticity using a micro-computed tomography image-processing method combined with nanoindentation. This analysis suggests that the regenerated bone demonstrates decreased elasticity compared with the uninjured bone, but there is no significant difference in elasticity between aged and young regenerated bone. These data highlight distinct architectural and biomechanical differences in regenerated bone in both young and aged mice and provide a new analysis tool for the digit amputation model to aid in evaluating the outcomes for potential therapeutic treatments to promote regeneration.
Original language | English |
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Article number | 749055 |
Journal | Frontiers in Cell and Developmental Biology |
Volume | 9 |
DOIs | |
State | Published - Oct 13 2021 |
Bibliographical note
Publisher Copyright:© Copyright © 2021 Hoffseth, Busse, Jaramillo, Simkin, Lacey and Sammarco.
Funding
This study was supported by a research grant from the National Institute of General Medical Sciences P20GM103629.
Funders | Funder number |
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National Institute of General Medical Sciences | P20GM103629 |
National Institute of General Medical Sciences |
Keywords
- aging
- biomechanics
- bone
- digit regeneration
- elastic modulus
- regeneration
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology