Age-related alterations in monoamine release from rat striatum: An in vivo electrochemical study

Greg M. Rose, Greg A. Gerhardt, Gary L. Conboy, Barry J. Hoffer

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

In vivo electrochemistry was used to examine presynaptic alterations in dopamine release in the striatum of aged rats. Chronoamperometric determinations of monoamine release, induced by local micro pressure-ejection of K+, were made using Nafron-coated graphite epoxy electrodes. Recordings were made from the striatum of urethane-anesthetized Fischer 344 rats at 6, 24, and ≥29 months of age. Following the in vivo electrochemical experiments, the animals were sacrificed and the caudate nucleus removed for analysis of whole tissue levels of monoamines and their metabolites using standard HPLC techniques. Overall, mean amplitudes of K+-evoked releases from the striatum of 6 month and 24 month F344 rats did not differ signficantly (p > 0.1). However, this result was complicated by the observation that the mean values obtained from two separate groups of 24 month animals, recorded 6 months apart, were significantly different from each other (p <0.001). Mean releases for the latter 24 month group were significantly less than for the 6 month group (p <0.01). No difference was found in the release magnitudes of 6 month animals recorded contemporaneously with the two groups of 24 month rats. Release amplitudes for the ≥29 month group were clearly less than from the 6 month animals (p <0.001). Both groups of 24 month rats, as well as the ≥29 month animals, also showed significant prolongation in the time courses of the K+-evoked releases when compared to the 6 month group (p <0.02 and p <0.01 respectively). Whole tissue levels of the monoamines and their principle metabolites did not differ between ages.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalNeurobiology of Aging
Volume7
Issue number2
DOIs
StatePublished - 1986

Bibliographical note

Funding Information:
Supported by USPHS grants AG-04418, NS-09199 and the Veterans Administration Medical Research Service.

Keywords

  • Dopamine
  • HPLC
  • In Vivo electrochemistry
  • Monoamines
  • Senescence
  • Striatum Aging

ASJC Scopus subject areas

  • Clinical Neurology
  • Geriatrics and Gerontology
  • Aging
  • General Neuroscience
  • Developmental Biology

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