TY - JOUR
T1 - Age-related changes in glutamate release in the CA3 and dentate gyrus of the rat hippocampus
AU - Stephens, Michelle L.
AU - Quintero, Jorge E.
AU - Pomerleau, Francois
AU - Huettl, Peter
AU - Gerhardt, Greg A.
PY - 2011/5
Y1 - 2011/5
N2 - The present studies employed a novel microelectrode array recording technology to study glutamate release and uptake in the dentate gyrus, CA3 and CA1 hippocampal subregions in anesthetized young, late-middle aged and aged male Fischer 344 rats. The mossy fiber terminals in CA3 showed a significantly decreased amount of KCl-evoked glutamate release in aged rats compared to both young and late-middle-aged rats. Significantly more KCl-evoked glutamate release was seen from perforant path terminals in the DG of late-middle-aged rats compared young and aged rats. The DG of aged rats developed an increased glutamate uptake rate compared to the DG of young animals, indicating a possible age-related change in glutamate regulation to deal with increased glutamate release that occurred in late-middle age. No age-related changes in resting levels of glutamate were observed in the DG, CA3 and CA1. Taken together, these data support dynamic changes to glutamate regulation during aging in subregions of the mammalian hippocampus that are critical for learning and memory.
AB - The present studies employed a novel microelectrode array recording technology to study glutamate release and uptake in the dentate gyrus, CA3 and CA1 hippocampal subregions in anesthetized young, late-middle aged and aged male Fischer 344 rats. The mossy fiber terminals in CA3 showed a significantly decreased amount of KCl-evoked glutamate release in aged rats compared to both young and late-middle-aged rats. Significantly more KCl-evoked glutamate release was seen from perforant path terminals in the DG of late-middle-aged rats compared young and aged rats. The DG of aged rats developed an increased glutamate uptake rate compared to the DG of young animals, indicating a possible age-related change in glutamate regulation to deal with increased glutamate release that occurred in late-middle age. No age-related changes in resting levels of glutamate were observed in the DG, CA3 and CA1. Taken together, these data support dynamic changes to glutamate regulation during aging in subregions of the mammalian hippocampus that are critical for learning and memory.
KW - Hippocampus
KW - L-Glutamate
KW - Voltammetry
UR - http://www.scopus.com/inward/record.url?scp=79954588006&partnerID=8YFLogxK
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U2 - 10.1016/j.neurobiolaging.2009.05.009
DO - 10.1016/j.neurobiolaging.2009.05.009
M3 - Article
C2 - 19535175
AN - SCOPUS:79954588006
SN - 0197-4580
VL - 32
SP - 811
EP - 820
JO - Neurobiology of Aging
JF - Neurobiology of Aging
IS - 5
ER -