To facilitate functional genomics in the soybean pathogen Phomopsis longicolla, we developed a robust Agrobacterium tumefaciens-mediated transformation system that yielded 150-250 transformants per 1×106 conidia of P. longicolla. This first report of P. longicolla transformation provides a useful tool for insertional mutagenesis in an increasingly important pathogen of soybean.
|Number of pages||2|
|Journal||Journal of Microbiological Methods|
|State||Published - Mar 2013|
Bibliographical noteFunding Information:
We thank Dr. Stephen Marek at Oklahoma State University for providing the A . tumefaciens strain AGL-1, and Dr. Thomas Mitchell at Ohio State University for providing the vector pBHt2_sGFP. This research was partially funded by the United Soybean Board (Project# 1261 ) and the USDA-ARS project 6402-21220-010-00 .
- Agrobacterium-mediated transformation
- GFP expression
- Phomopsis longicolla
- Phomopsis seed decay
ASJC Scopus subject areas
- Molecular Biology
- Microbiology (medical)