Agrobacterium tumefaciens-mediated transformation of the soybean pathogen Phomopsis longicolla

Shuxian Li, John B. Ridenour, Hun Kim, Robert L. Hirsch, John C. Rupe, Burton H. Bluhm

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

To facilitate functional genomics in the soybean pathogen Phomopsis longicolla, we developed a robust Agrobacterium tumefaciens-mediated transformation system that yielded 150-250 transformants per 1×106 conidia of P. longicolla. This first report of P. longicolla transformation provides a useful tool for insertional mutagenesis in an increasingly important pathogen of soybean.

Original languageEnglish
Pages (from-to)244-245
Number of pages2
JournalJournal of Microbiological Methods
Volume92
Issue number3
DOIs
StatePublished - Mar 2013

Bibliographical note

Funding Information:
We thank Dr. Stephen Marek at Oklahoma State University for providing the A . tumefaciens strain AGL-1, and Dr. Thomas Mitchell at Ohio State University for providing the vector pBHt2_sGFP. This research was partially funded by the United Soybean Board (Project# 1261 ) and the USDA-ARS project 6402-21220-010-00 .

Keywords

  • Agrobacterium-mediated transformation
  • GFP expression
  • Phomopsis longicolla
  • Phomopsis seed decay

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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