Agrobacterium tumefaciens-mediated transformation of the soybean pathogen Phomopsis longicolla

Shuxian Li; John B. Ridenour; Hun Kim; Robert L. Hirsch; John C. Rupe; Burton H. Bluhm

doi:10.1016/j.mimet.2012.12.015

Agrobacterium tumefaciens-mediated transformation of the soybean pathogen Phomopsis longicolla

Shuxian Li, John B. Ridenour, Hun Kim, Robert L. Hirsch, John C. Rupe, Burton H. Bluhm

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

To facilitate functional genomics in the soybean pathogen Phomopsis longicolla, we developed a robust Agrobacterium tumefaciens-mediated transformation system that yielded 150-250 transformants per 1×10⁶ conidia of P. longicolla. This first report of P. longicolla transformation provides a useful tool for insertional mutagenesis in an increasingly important pathogen of soybean.

Original language	English
Pages (from-to)	244-245
Number of pages	2
Journal	Journal of Microbiological Methods
Volume	92
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2012.12.015
State	Published - Mar 2013

Bibliographical note

Funding Information:
We thank Dr. Stephen Marek at Oklahoma State University for providing the A . tumefaciens strain AGL-1, and Dr. Thomas Mitchell at Ohio State University for providing the vector pBHt2_sGFP. This research was partially funded by the United Soybean Board (Project# 1261 ) and the USDA-ARS project 6402-21220-010-00 .

Funding

We thank Dr. Stephen Marek at Oklahoma State University for providing the A . tumefaciens strain AGL-1, and Dr. Thomas Mitchell at Ohio State University for providing the vector pBHt2_sGFP. This research was partially funded by the United Soybean Board (Project# 1261 ) and the USDA-ARS project 6402-21220-010-00 .

Funders	Funder number
USDA-ARS	6402-21220-010-00
United Soybean Board	1261

Keywords

Agrobacterium-mediated transformation
GFP expression
Phomopsis longicolla
Phomopsis seed decay

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2012.12.015

Cite this

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title = "Agrobacterium tumefaciens-mediated transformation of the soybean pathogen Phomopsis longicolla",

abstract = "To facilitate functional genomics in the soybean pathogen Phomopsis longicolla, we developed a robust Agrobacterium tumefaciens-mediated transformation system that yielded 150-250 transformants per 1×106 conidia of P. longicolla. This first report of P. longicolla transformation provides a useful tool for insertional mutagenesis in an increasingly important pathogen of soybean.",

keywords = "Agrobacterium-mediated transformation, GFP expression, Phomopsis longicolla, Phomopsis seed decay",

author = "Shuxian Li and Ridenour, \{John B.\} and Hun Kim and Hirsch, \{Robert L.\} and Rupe, \{John C.\} and Bluhm, \{Burton H.\}",

note = "Funding Information: We thank Dr. Stephen Marek at Oklahoma State University for providing the A . tumefaciens strain AGL-1, and Dr. Thomas Mitchell at Ohio State University for providing the vector pBHt2\_sGFP. This research was partially funded by the United Soybean Board (Project\# 1261 ) and the USDA-ARS project 6402-21220-010-00 . ",

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doi = "10.1016/j.mimet.2012.12.015",

language = "English",

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T1 - Agrobacterium tumefaciens-mediated transformation of the soybean pathogen Phomopsis longicolla

AU - Li, Shuxian

AU - Ridenour, John B.

AU - Kim, Hun

AU - Hirsch, Robert L.

AU - Rupe, John C.

AU - Bluhm, Burton H.

N1 - Funding Information: We thank Dr. Stephen Marek at Oklahoma State University for providing the A . tumefaciens strain AGL-1, and Dr. Thomas Mitchell at Ohio State University for providing the vector pBHt2_sGFP. This research was partially funded by the United Soybean Board (Project# 1261 ) and the USDA-ARS project 6402-21220-010-00 .

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N2 - To facilitate functional genomics in the soybean pathogen Phomopsis longicolla, we developed a robust Agrobacterium tumefaciens-mediated transformation system that yielded 150-250 transformants per 1×106 conidia of P. longicolla. This first report of P. longicolla transformation provides a useful tool for insertional mutagenesis in an increasingly important pathogen of soybean.

AB - To facilitate functional genomics in the soybean pathogen Phomopsis longicolla, we developed a robust Agrobacterium tumefaciens-mediated transformation system that yielded 150-250 transformants per 1×106 conidia of P. longicolla. This first report of P. longicolla transformation provides a useful tool for insertional mutagenesis in an increasingly important pathogen of soybean.

KW - Agrobacterium-mediated transformation

KW - GFP expression

KW - Phomopsis longicolla

KW - Phomopsis seed decay

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Agrobacterium tumefaciens-mediated transformation of the soybean pathogen Phomopsis longicolla

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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