The alteration of microRNA (miRNA) expression plays an important role in chemical carcinogenesis. Presently, few reports have been published that concern the significance of circulating miRNAs in lung carcinogenesis induced by environmental carcinogens. The purpose of this study was to identify serum miRNAs that could be associated with lung carcinogenesis induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Male F344 rats were systemically administered with NNK. The rat serum differential expression profiles of miRNAs were analyzed by small RNA solexa sequencing. Using quantitative real-time PCR, the differentially expressed serum miRNAs were identified in each individual rat. Serum miR-206 and miR-133b were selected for further identification in rat serum at different stages of lung carcinogenesis; we detected the levels of serum miR-206 and miR-133b in lung cancer tissues induced by NNK. NNK causes significant alteration of serum miRNA expression. Compared to the control group, serum miR-206 and miR-133b were significantly up-regulated in the early stage of NNK-induced lung carcinogenesis. miR-206 and miR-133b exhibited low-expression in lung cancer tissues. Our results demonstrate that lung carcinogen NNK exposure changes the expression of serum miRNAs. Serum miR-206 and miR-133b could be associated with lung carcinogenesis induced by NNK.
|Number of pages||9|
|Journal||Toxicology and Applied Pharmacology|
|State||Published - Mar 5 2013|
Bibliographical noteFunding Information:
The authors thank Dr. Xiaoming Zhou for the expert assistance with pathology analysis. We also thank Beijing Genomics Institute at Shenzhen (BGI Shenzhen, China) for the assistance with small RNA solexa sequencing. This work was supported by the National Key Basic Research Program of China ( 2012CB525004 to J.Y.), the National Natural Science Foundation of China ( 30972443 to J.Y.), the Research Fund for Doctoral Program of Higher Education of China ( 20114423110002 to J.Y.), the Key Program of Guangdong Natural Science Foundation ( 9251018201000004 to J.Y.), the University Talent Program of Guangdong ( 2010-79 to J.Y.), the University Talent Program of Guangzhou ( 10A003D to J.Y.), the Science and Technology Project of Guangzhou ( 2010Y1-C441 to J.Y.), the Youth Foundation of China State Key Laboratory of Respiratory Disease ( 201105 to W.J.) and the Medical Scientific Research Foundation of Guangdong Province ( A2011234 to W.J.)
- Lung carcinogenesis
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