Abstract
The fibrinolytic enzyme profile of SMS-KAN human neuroblastoma cells was found to vary dramatically during the differentiation process. Five maturational agents - retinoic acid, dibutyryl cAMP, 5-bromodeoxyuridine, sodium butyrate and phorbol myristate acetate were tested for their effects on cellular morphology, DNA synthesis, plasminogen activator (PA) and PA inhibitor (PAI) activity. SMS-KAN cells secrete urokinase (UK) and tissue PA (tPA) as well as a possibly unique PAI. Treatment of cells with 1 μM RA resulted in an inhibition of proliferation, extension of neurite-like processes indicative of differentiation, as well as a switch from secretion of UK to tPA and a reduction in PAI secretion. Other agents which caused neural process formation and decreased cell proliferation also induced alterations in PA PAI while agents which had no detectable effect on cell growth induced little change in the fibrinolytic enzyme profile.
| Original language | English |
|---|---|
| Pages (from-to) | 101-108 |
| Number of pages | 8 |
| Journal | Cancer Letters |
| Volume | 44 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1989 |
Funding
This investigation was supported by the National Cancer Institute of Canada (R.C.M.), the Alberta Cancer Board (D.A.H.) and the Alberta Heritage Foundation for Medical Research (AHFMR). L.A.B. was supported by an AHFMR Studentship and R.C.M. and D.A.H. are AHFMR Scholars.
| Funders | Funder number |
|---|---|
| National Cancer Institute of Canada | |
| Alberta Cancer Board | |
| Alberta Heritage Foundation for Medical Research Studentship |
Keywords
- differentiation
- neurite outgrowth
- neuroblastoma
- plasminogen activator
- plasminogen activator inhibitor
- tissue-type PA
- urokinase
ASJC Scopus subject areas
- Oncology
- Cancer Research
