Alterations in the balance of protein kinase/phosphatase activities parallel reduced synaptic strength during aging

Christopher M. Norris; Shelley Halpain; Thomas C. Foster

doi:10.1152/jn.1998.80.3.1567

Alterations in the balance of protein kinase/phosphatase activities parallel reduced synaptic strength during aging

Christopher M. Norris, Shelley Halpain, Thomas C. Foster

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Abstract

The current research examined the regulation of synaptic strength by protein phosphorylation during aging. Bath application of the protein phosphatase 1 and 2A (PP1 and PP2A) inhibitor calyculin A (1 μM) enhanced CA3-CA1 synaptic strength in hippocampal slices from aged male (20-24 mo) but not from young adult male (4-6 mo) Fischer 344 rats. Similarly, injection of the PP1 and PP2A inhibitor microcystin-L,R (5 μM) into CA1 cells caused an increase in the intracellular synaptic response only in slices from aged rats. In contrast, bath application of the serine/threonine kinase inhibitor H-7 (10 μM) induced a decrease in synaptic strength only in slices from the young adult group. These results demonstrate that phosphorylation-dependent regulation of intrinsic synaptic efficacy changes during aging.

Original language	English
Pages (from-to)	1567-1570
Number of pages	4
Journal	Journal of Neurophysiology
Volume	80
Issue number	3
DOIs	https://doi.org/10.1152/jn.1998.80.3.1567
State	Published - Sep 1998

ASJC Scopus subject areas

Neuroscience (all)
Physiology

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10.1152/jn.1998.80.3.1567

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abstract = "The current research examined the regulation of synaptic strength by protein phosphorylation during aging. Bath application of the protein phosphatase 1 and 2A (PP1 and PP2A) inhibitor calyculin A (1 μM) enhanced CA3-CA1 synaptic strength in hippocampal slices from aged male (20-24 mo) but not from young adult male (4-6 mo) Fischer 344 rats. Similarly, injection of the PP1 and PP2A inhibitor microcystin-L,R (5 μM) into CA1 cells caused an increase in the intracellular synaptic response only in slices from aged rats. In contrast, bath application of the serine/threonine kinase inhibitor H-7 (10 μM) induced a decrease in synaptic strength only in slices from the young adult group. These results demonstrate that phosphorylation-dependent regulation of intrinsic synaptic efficacy changes during aging.",

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Alterations in the balance of protein kinase/phosphatase activities parallel reduced synaptic strength during aging

Abstract

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