The interaction among heart failure (HF), chronic kidney disease (CKD), and anemia is called cardio-renal anemia syndrome. The mechanism of anemia in cardio-renal anemia syndrome is complex and remains completely unknown. We have previously reported that impaired intestinal iron transporters may contribute to the mechanism of anemia in HF using in vivo HF model rats. In this study, we assessed intestinal iron transporters in CKD model rats to investigate the association of intestinal iron transporters in the mechanism of cardio-renal anemia syndrome. CKD was induced by 5/6 nephrectomy in Sprague–Dawley rats. Sham-operated rats served as a control. After 24-week surgery, CKD rats exhibited normocytic normochromic anemia and normal serum erythropoietin levels despite of anemia. Serum iron levels were decreased in CKD rats compared with the controls. Of interest, intestinal expression of critical iron importers, such as duodenal cytochrome b (Dcyt-b) and divalent metal transporter 1 (DMT-1), was decreased in CKD rats compared with the controls. On the other hand, intestinal expression of ferroportin, an intestinal iron exporter, was not different in the control and CKD groups. Moreover, hepatic expression of hepcidin, a regulator of iron homeostasis, did not differ between the control and CKD groups. These results suggest that impaired intestinal expression of Dcyt-b and DMT-1 might be associated with the reduction of an iron uptake in CKD. Taken together, impaired these intestinal iron transporters may become a novel therapeutic target for cardio-renal anemia syndrome.
|Number of pages
|Heart and Vessels
|Published - Nov 1 2017
Bibliographical noteFunding Information:
Acknowledgements We gratefully thank Sachi Ito for technical assistance. This study was supported by a Grant-in-Aid for Scientific Research (C) (JSPS KAKENHI Grant Nos. 25460919 and 16K09273) nostaining of the intestine sections and hepcidin-25 immunostain-ing of the liver sections in the control and CKD groups. Scale bars 50 µm. *p < 0.05 versus control group and grants from The Salt Science Research Foundation (Nos. 1544 and 1641), Suzuken Memorial Foundation, Takeda Science Foundation, and The Vehicle Racing Commemorative Foundation (to Y. Naito). This study was also supported by a Grant-in-Aid for Scientific Research (C) (JSPS KAKENHI Grant No. 24590907 to T. Masuyama and No. 15K09148 to T. Tsujino).
© 2017, Springer Japan KK.
- Cardio-renal anemia syndrome
- Divalent metal transporter 1
- Duodenal cytochrome b
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine