Altered Phenylpropanoid Metabolism in the Maize Lc-Expressed Sweet Potato (Ipomoea batatas) Affects Storage Root Development

Hongxia Wang, Jun Yang, Min Zhang, Weijuan Fan, Nurit Firon, Sitakanta Pattanaik, Ling Yuan, Peng Zhang

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40 Scopus citations

Abstract

There is no direct evidence of the effect of lignin metabolism on early storage root development in sweet potato. In this study, we found that heterologous expression of the maize leaf color (Lc) gene in sweet potato increased anthocyanin pigment accumulation in the whole plant and resulted in reduced size with an increased length/width ratio, low yield and less starch content in the early storage roots. RT-PCR analysis revealed dramatic up-regulation of the genes involved in the lignin biosynthesis pathway in developing storage roots, leading to greater lignin content in the Lc transgenic lines, compared to the wild type. This was also evidenced by the enhanced lignification of vascular cells in the early storage roots. Furthermore, increased expression of the β-amylase gene in leaves and storage roots also accelerated starch degradation and increased the sugar use efficiency, providing more energy and carbohydrate sources for lignin biosynthesis in the Lc transgenic sweet potato. Lesser starch accumulation was observed in the developing storage roots at the initiation stage in the Lc plants. Our study provides experimental evidence of the basic carbohydrate metabolism underlying the development of storage roots, which is the transformation of lignin biosynthesis to starch biosynthesis.

Original languageEnglish
Article number18645
JournalScientific Reports
Volume6
DOIs
StatePublished - Jan 4 2016

Bibliographical note

Funding Information:
We thank Prof. Laigeng Li from SIPPE, CAS for the technical advice and assistance, Dr. Yining Liu for HPLC-MS-MS analysis and Mr. Xiaoyan Gao for scanning electron microscopy. This work was supported by grants from the National High Technology Research and Development Program of China (No. 2012AA101204, 2011AA100607), the International Science and Technology Cooperation Program of China (No. 2015DFG32370), the National Natural Science Foundation of China (31201254), the Science and Technology Commission of Shanghai Municipality (14DZ 2260400) and Shanghai Municipal Afforestation & City Appearance and Environmental Sanitation Administration (F132427, F122422).

ASJC Scopus subject areas

  • General

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