Alternative processing of the sarco/endoplasmic reticulum Ca2+-ATPase transcripts during muscle differentiation is a specifically regulated process

Ludo Van Den Bosch, Luc Mertens, Yvon Cavaloc, Martha Peterson, Frank Wuytack, Jan Eggermont

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Expression of the muscle-specific 2a isoform of the sarco/ endoplasmic reticulum Ca2+-ATPase (SERCA2) requires activation of an otherwise inefficient splice process at the 3′-end of the primary gene transcript. We provide evidence that SERCA2 splicing is a specifically regulated process, rather than the result of an increase in general splice efficiency or a decrease in polyadenylation efficiency at the 5′-most polyadenylation site. This is indicated by the fact that changes in general splice and polyadenylation efficiency, as observed during B-cell maturation, did not affect SERCA2 splicing. Furthermore, expression and overexpression studies did not support the hypothesis that changes in the level of the alternative splice factor ASF/SF2 or other arginine and serine rich proteins are sufficient to obtain the regulation of muscle- and neuronal-specific splicing.

Original languageEnglish
Pages (from-to)647-651
Number of pages5
JournalBiochemical Journal
Volume317
Issue number3
DOIs
StatePublished - Aug 1 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Alternative processing of the sarco/endoplasmic reticulum Ca2+-ATPase transcripts during muscle differentiation is a specifically regulated process'. Together they form a unique fingerprint.

Cite this