Nanotechnology is a rapidly growing industry that has elicited much concern because of the lack of available toxicity data. Exposure to ultrafine particles may be a risk for the development of vascular diseases due to dysfunction of the vascular endothelium. Increased endothelial adhesiveness is a critical first step in the development of vascular diseases, such as atherosclerosis. The hypothesis that alumina nanoparticles increase inflammatory markers of the endothelium, measured by the induction of adhesion molecules as well as the adhesion of monocytes to the endothelial monolayer, was tested. Following characterization of alumina nanoparticles by transmission electron microscopy (TEM), electron diffraction, and particle size distribution analysis, endothelial cells were exposed to alumina at various concentrations and times. Both porcine pulmonary artery endothelial cells and human umbilical vein endothelial cells showed increased mRNA and protein expression of VCAM-1, ICAM-1, and ELAM-1. Furthermore, human endothelial cells treated with alumina particles showed increased adhesion of activated monocytes. The alumina particles tended to agglomerate at physiological pH in serum-containing media, which led to a range of particle sizes from nano to micron size during treatment conditions. These data show that alumina nanoparticles can elicit a proinflammatory response and thus present a cardiovascular disease risk.
|Number of pages||7|
|State||Published - May 30 2008|
Bibliographical noteFunding Information:
We would like to thank Dr. Thomas Curry for help obtaining human umbilical cords. This research was supported by grants from NIEHS/NIH (P42ES07380), AHA Pre-doctoral Fellowship (0613216B), the University of Kentucky Research Support Fund, and the University of Kentucky Agricultural Experiment Station.
- Adhesion molecules
- Cardiovascular disease
- Manufactured nanoparticles
ASJC Scopus subject areas