Amino acid residue-specific stable isotope labeling for quantitative proteomics

A residue specific isotope labeling technique was developed in which deuterium enriched amino acids are incorporated into proteins during cell growth. This technique can be used to quantify protein relative abundance in different cell populations. S. cerevisiae strain DY 1457 was grown in synthetic defined (SD) medium containing either normal Leu or the deuterium enriched Leu-d3. The cell pellets harvested during log phase was washed with water and suspended in lysis buffer. Six peaks with the characteristic 3Da splitting pattern were obtained.