Ammonia permeability of the soybean nodulin 26 channel

Jin Ha Hwang, Sally R. Ellingson, Daniel M. Roberts

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Soybean nodulin 26 (nod26), a member of the aquaporin superfamily, is the major protein component of the symbiosome membrane that encloses nitrogen-fixing bacteroids in root nodules. Previous work has demonstrated that nod26 facilitates the transport of water and glycerol, although a potential additional role as a channel for fixed ammonia efflux has been hypothesized. In the present study it is shown that recombinant nod26 reconstituted into proteoliposomes facilitates NH3 transport in an Hg2+-sensitive manner with a reduced activation energy, hallmarks of protein-facilitated transport characteristic of aquaporins. Comparison of the predicted single-channel transport rates of nod26 suggests a 4.9-fold preference for ammonia compared to water.

Original languageEnglish
Pages (from-to)4339-4343
Number of pages5
JournalFEBS Letters
Volume584
Issue number20
DOIs
StatePublished - 2010

Bibliographical note

Funding Information:
Supported by National Science Foundation Grant MCB-0618075 to DMR.

Keywords

  • Aquaporin
  • Legume
  • Nitrogen fixation
  • Symbiosis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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