Amperometric self-referencing ceramic based microelectrode arrays for D-serine detection

Diana Campos-Beltrán, Åsa Konradsson-Geuken, Jorge E. Quintero, Lisa Marshall

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO).We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 ± 0.83 pA/μM to D-serine, a limit of detection (LOD) of 0.17 ± 0.01 μM, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean ± SEM; n = 12) that can be used for freely moving studies.

Original languageEnglish
Article number20
JournalBiosensors
Volume8
Issue number1
DOIs
StatePublished - Mar 6 2018

Bibliographical note

Funding Information:
Acknowledgments: We thank Sonja Binder, Katia Monsorno, Ines Stölting, Horst Koller, Andreas Moser and Jose Ostos Muraday for technical assistance and advice. This work was supported by Deutsche Forschungsgemeinschaft SFBTR654 Plasticity and Sleep (TPA06) and Deutsche Forschungsgemeinschaft SPP1665 (MA2053/4-2).

Publisher Copyright:
© 2018 by the authors.

Keywords

  • Amperometry
  • Biosensor
  • D-serine
  • Microelectrode array
  • Self-referencing

ASJC Scopus subject areas

  • Analytical Chemistry
  • Instrumentation
  • Engineering (miscellaneous)
  • Biotechnology
  • Biomedical Engineering
  • Clinical Biochemistry

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