Amperometric self-referencing ceramic based microelectrode arrays for D-serine detection

Diana Campos-Beltrán; Åsa Konradsson-Geuken; Jorge E. Quintero; Lisa Marshall

doi:10.3390/bios8010020

Amperometric self-referencing ceramic based microelectrode arrays for D-serine detection

Diana Campos-Beltrán, Åsa Konradsson-Geuken, Jorge E. Quintero, Lisa Marshall

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO).We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 ± 0.83 pA/μM to D-serine, a limit of detection (LOD) of 0.17 ± 0.01 μM, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean ± SEM; n = 12) that can be used for freely moving studies.

Original language	English
Article number	20
Journal	Biosensors
Volume	8
Issue number	1
DOIs	https://doi.org/10.3390/bios8010020
State	Published - Mar 6 2018

Bibliographical note

Funding Information:
Acknowledgments: We thank Sonja Binder, Katia Monsorno, Ines Stölting, Horst Koller, Andreas Moser and Jose Ostos Muraday for technical assistance and advice. This work was supported by Deutsche Forschungsgemeinschaft SFBTR654 Plasticity and Sleep (TPA06) and Deutsche Forschungsgemeinschaft SPP1665 (MA2053/4-2).

Publisher Copyright:
© 2018 by the authors.

Keywords

Amperometry
Biosensor
D-serine
Microelectrode array
Self-referencing

ASJC Scopus subject areas

Clinical Biochemistry

Access to Document

10.3390/bios8010020

Cite this

@article{861c837bcd2c4e2ea51531e4fa16294f,

title = "Amperometric self-referencing ceramic based microelectrode arrays for D-serine detection",

abstract = "D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO).We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 ± 0.83 pA/μM to D-serine, a limit of detection (LOD) of 0.17 ± 0.01 μM, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean ± SEM; n = 12) that can be used for freely moving studies.",

keywords = "Amperometry, Biosensor, D-serine, Microelectrode array, Self-referencing",

author = "Diana Campos-Beltr{\'a}n and {\AA}sa Konradsson-Geuken and Quintero, {Jorge E.} and Lisa Marshall",

note = "Funding Information: Acknowledgments: We thank Sonja Binder, Katia Monsorno, Ines St{\"o}lting, Horst Koller, Andreas Moser and Jose Ostos Muraday for technical assistance and advice. This work was supported by Deutsche Forschungsgemeinschaft SFBTR654 Plasticity and Sleep (TPA06) and Deutsche Forschungsgemeinschaft SPP1665 (MA2053/4-2). Publisher Copyright: {\textcopyright} 2018 by the authors.",

year = "2018",

month = mar,

day = "6",

doi = "10.3390/bios8010020",

language = "English",

volume = "8",

number = "1",

}

TY - JOUR

T1 - Amperometric self-referencing ceramic based microelectrode arrays for D-serine detection

AU - Campos-Beltrán, Diana

AU - Konradsson-Geuken, Åsa

AU - Quintero, Jorge E.

AU - Marshall, Lisa

N1 - Funding Information: Acknowledgments: We thank Sonja Binder, Katia Monsorno, Ines Stölting, Horst Koller, Andreas Moser and Jose Ostos Muraday for technical assistance and advice. This work was supported by Deutsche Forschungsgemeinschaft SFBTR654 Plasticity and Sleep (TPA06) and Deutsche Forschungsgemeinschaft SPP1665 (MA2053/4-2). Publisher Copyright: © 2018 by the authors.

PY - 2018/3/6

Y1 - 2018/3/6

N2 - D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO).We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 ± 0.83 pA/μM to D-serine, a limit of detection (LOD) of 0.17 ± 0.01 μM, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean ± SEM; n = 12) that can be used for freely moving studies.

AB - D-serine is the major D-amino acid in the mammalian central nervous system. As the dominant co-agonist of the endogenous synaptic NMDA receptor, D-serine plays a role in synaptic plasticity, learning, and memory. Alterations in D-serine are linked to neuropsychiatric disorders including schizophrenia. Thus, it is of increasing interest to monitor the concentration of D-serine in vivo as a relevant player in dynamic neuron-glia network activity. Here we present a procedure for amperometric detection of D-serine with self-referencing ceramic-based microelectrode arrays (MEAs) coated with D-amino acid oxidase from the yeast Rhodotorula gracilis (RgDAAO).We demonstrate in vitro D-serine recordings with a mean sensitivity of 8.61 ± 0.83 pA/μM to D-serine, a limit of detection (LOD) of 0.17 ± 0.01 μM, and a selectivity ratio of 80:1 or greater for D-serine over ascorbic acid (mean ± SEM; n = 12) that can be used for freely moving studies.

KW - Amperometry

KW - Biosensor

KW - D-serine

KW - Microelectrode array

KW - Self-referencing

UR - http://www.scopus.com/inward/record.url?scp=85043376106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043376106&partnerID=8YFLogxK

U2 - 10.3390/bios8010020

DO - 10.3390/bios8010020

M3 - Article

C2 - 29509674

AN - SCOPUS:85043376106

VL - 8

IS - 1

M1 - 20

ER -

Amperometric self-referencing ceramic based microelectrode arrays for D-serine detection

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this