Amplicon sequencing of 42 nuclear loci supports directional gene flow between South Pacific populations of a hydrothermal vent limpet

Sophie Plouviez, Abigail Leavitt LaBella, David W. Weisrock, F. A.Bastiaan von Meijenfeldt, Bernard Ball, Joseph E. Neigel, Cindy L. Van Dover

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

In the past few decades, population genetics and phylogeographic studies have improved our knowledge of connectivity and population demography in marine environments. Studies of deep-sea hydrothermal vent populations have identified barriers to gene flow, hybrid zones, and demographic events, such as historical population expansions and contractions. These deep-sea studies, however, used few loci, which limit the amount of information they provided for coalescent analysis and thus our ability to confidently test complex population dynamics scenarios. In this study, we investigated population structure, demographic history, and gene flow directionality among four Western Pacific hydrothermal vent populations of the vent limpet Lepetodrilus aff. schrolli. These vent sites are located in the Manus and Lau back-arc basins, currently of great interest for deep-sea mineral extraction. A total of 42 loci were sequenced from each individual using high-throughput amplicon sequencing. Amplicon sequences were analyzed using both genetic variant clustering methods and evolutionary coalescent approaches. Like most previously investigated vent species in the South Pacific, L. aff. schrolli showed no genetic structure within basins but significant differentiation between basins. We inferred significant directional gene flow from Manus Basin to Lau Basin, with low to no gene flow in the opposite direction. This study is one of the very few marine population studies using >10 loci for coalescent analysis and serves as a guide for future marine population studies.

Original languageEnglish
Pages (from-to)6568-6580
Number of pages13
JournalEcology and Evolution
Volume9
Issue number11
DOIs
StatePublished - Jun 2019

Bibliographical note

Publisher Copyright:
© 2019 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

Keywords

  • conservation genetics
  • deep-sea
  • invertebrates
  • phylogeography
  • population genetics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Nature and Landscape Conservation

Fingerprint

Dive into the research topics of 'Amplicon sequencing of 42 nuclear loci supports directional gene flow between South Pacific populations of a hydrothermal vent limpet'. Together they form a unique fingerprint.

Cite this