Amputation-induced reactive oxygen species signaling is required for axolotl tail regeneration

Nour W. Al Haj Baddar, Adarsh Chithrala, S. Randal Voss

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Background: Among vertebrates, salamanders are unparalleled in their ability to regenerate appendages throughput life. However, little is known about early signals that initiate regeneration in salamanders. Results: Ambystoma mexicanum embryos were administered tail amputations to investigate the timing of reactive oxygen species (ROS) production and the requirement of ROS for regeneration. ROS detected by dihydroethidium increased within minutes of axolotl tail amputation and levels remained high for 24 hr. Pharmacological inhibition of ROS producing enzymes with diphenyleneiodonium chloride (DPI) and VAS2870 reduced ROS levels. Furthermore, DPI treatment reduced cellular proliferation and inhibited tail outgrowth. Conclusions: The results show that ROS levels increase in response to injury and are required for tail regeneration. These findings suggest that ROS provide instructive, if not initiating cues, for salamander tail regeneration. Developmental Dynamics 248:189-196, 2019.

Original languageEnglish
Pages (from-to)189-196
Number of pages8
JournalDevelopmental Dynamics
Issue number2
StatePublished - Feb 2019

Bibliographical note

Publisher Copyright:
© 2018 Wiley Periodicals, Inc.


  • Ambystoma mexicanum
  • ROS
  • axolotl
  • regeneration
  • superoxide anion

ASJC Scopus subject areas

  • Developmental Biology


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