TY - JOUR
T1 - An Aminopeptidase from Bovine Brain Which Catalyzes the Hydrolysis of Enkephalin
AU - Hersh, Louis B.
AU - McKelvy, Jeffrey F.
PY - 1981/1
Y1 - 1981/1
N2 - An aminopeptidase from bovine brain which catalyzes the hydrolysis of the tyrosyl1‐glycine2 bond of methionine5‐enkephalin has been purified to electrophoretic homogeneity. The enzyme also catalyzes the hydrolysis of di‐peptides, tripeptides, and amino acid β‐naphthylamides. The enzyme can be inactivated by dialysis against EDTA, and reconstituted with divalent metal ions. Inhibition of the enzyme is observed in the presence of p‐chloromercuribenzoate and puromycin, the latter compound not being hydro‐lyzed by the enzyme. The enzyme is composed of a single polypeptide chain of molecular weight approx. 100,000. The properties of this enzyme are similar to those reported for other brain aminopeptidases active on enkephalin, although distinct differences are observed.
AB - An aminopeptidase from bovine brain which catalyzes the hydrolysis of the tyrosyl1‐glycine2 bond of methionine5‐enkephalin has been purified to electrophoretic homogeneity. The enzyme also catalyzes the hydrolysis of di‐peptides, tripeptides, and amino acid β‐naphthylamides. The enzyme can be inactivated by dialysis against EDTA, and reconstituted with divalent metal ions. Inhibition of the enzyme is observed in the presence of p‐chloromercuribenzoate and puromycin, the latter compound not being hydro‐lyzed by the enzyme. The enzyme is composed of a single polypeptide chain of molecular weight approx. 100,000. The properties of this enzyme are similar to those reported for other brain aminopeptidases active on enkephalin, although distinct differences are observed.
KW - Aminopeptidase
KW - Enkephalin hydrolysis
KW - Properties
KW - Purification
KW - Substrate specificity
UR - https://www.scopus.com/pages/publications/0019432699
UR - https://www.scopus.com/inward/citedby.url?scp=0019432699&partnerID=8YFLogxK
U2 - 10.1111/j.1471-4159.1981.tb02392.x
DO - 10.1111/j.1471-4159.1981.tb02392.x
M3 - Article
C2 - 6780659
AN - SCOPUS:0019432699
SN - 0022-3042
VL - 36
SP - 171
EP - 178
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 1
ER -