An easy-to-use tool for planning and modeling a calorimetric titration

Tapan Biswas, Oleg V. Tsodikov

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Isothermal titration calorimetry (ITC) is widely employed to measure thermodynamic properties of binding interactions between two macromolecules or a macromolecule and a small ligand. No labeling of interacting species is required for ITC, but this advantage is offset by potentially material-consuming experimental optimization complicated by an indirect readout of an ITC titration. Here we present a simple, practical, and portable spreadsheet-based tool for planning and modeling an ITC titration experiment accompanied by basic guidelines.

Original languageEnglish
Pages (from-to)91-93
Number of pages3
JournalAnalytical Biochemistry
Volume406
Issue number1
DOIs
StatePublished - Nov 2010

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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