Polyclonal antibodies against bovine milk lipoprotein lipase (LPL) were used to generate an enzyme-linked immunosorbent assay (ELISA) for rat LPL. The antibodies to LPL were affinity purified on bovine LPL columns and were shown to be specific for LPL by immunoprecipitation and enzyme inhibition. The solid-phase ELISA was sensitive from 1.0 to 20 ng/ml of LPL and paralleled enzyme activity. Denatured rat LPL showed the same LPL mass as undenatured samples, allowing LPL mass to be quantitated effectively in a variety of rat tissue extracts.
|Number of pages||9|
|State||Published - Oct 1987|
Bibliographical noteFunding Information:
This work was supported by the National Institutes of Health (HL2848 I, AM-HL 37085), the American Heart Association. Greater Los Angeles Affiliate (4921G 13). and the Veterans Administration. The authors also are indebted to Arlene S. Garfinkel for her help in assembling the manuscript.
- lipoprotein lipase
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology