An enzyme-linked immunoassay for lipoprotein lipase

John W.F. Goers; Mary E. Pedersen; Philip A. Kern; John Ong; Michael C. Schotz

doi:10.1016/0003-2697(87)90541-0

An enzyme-linked immunoassay for lipoprotein lipase

John W.F. Goers, Mary E. Pedersen, Philip A. Kern, John Ong, Michael C. Schotz

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Polyclonal antibodies against bovine milk lipoprotein lipase (LPL) were used to generate an enzyme-linked immunosorbent assay (ELISA) for rat LPL. The antibodies to LPL were affinity purified on bovine LPL columns and were shown to be specific for LPL by immunoprecipitation and enzyme inhibition. The solid-phase ELISA was sensitive from 1.0 to 20 ng/ml of LPL and paralleled enzyme activity. Denatured rat LPL showed the same LPL mass as undenatured samples, allowing LPL mass to be quantitated effectively in a variety of rat tissue extracts.

Original language	English
Pages (from-to)	27-35
Number of pages	9
Journal	Analytical Biochemistry
Volume	166
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(87)90541-0
State	Published - Oct 1987

Bibliographical note

Funding Information:
This work was supported by the National Institutes of Health (HL2848 I, AM-HL 37085), the American Heart Association. Greater Los Angeles Affiliate (4921G 13). and the Veterans Administration. The authors also are indebted to Arlene S. Garfinkel for her help in assembling the manuscript.

Keywords

ELISA
bovine
immunoassay
lipoprotein lipase
rat

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0003-2697(87)90541-0

Cite this

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abstract = "Polyclonal antibodies against bovine milk lipoprotein lipase (LPL) were used to generate an enzyme-linked immunosorbent assay (ELISA) for rat LPL. The antibodies to LPL were affinity purified on bovine LPL columns and were shown to be specific for LPL by immunoprecipitation and enzyme inhibition. The solid-phase ELISA was sensitive from 1.0 to 20 ng/ml of LPL and paralleled enzyme activity. Denatured rat LPL showed the same LPL mass as undenatured samples, allowing LPL mass to be quantitated effectively in a variety of rat tissue extracts.",

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note = "Funding Information: This work was supported by the National Institutes of Health (HL2848 I, AM-HL 37085), the American Heart Association. Greater Los Angeles Affiliate (4921G 13). and the Veterans Administration. The authors also are indebted to Arlene S. Garfinkel for her help in assembling the manuscript.",

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AU - Pedersen, Mary E.

AU - Kern, Philip A.

AU - Ong, John

AU - Schotz, Michael C.

N1 - Funding Information: This work was supported by the National Institutes of Health (HL2848 I, AM-HL 37085), the American Heart Association. Greater Los Angeles Affiliate (4921G 13). and the Veterans Administration. The authors also are indebted to Arlene S. Garfinkel for her help in assembling the manuscript.

PY - 1987/10

Y1 - 1987/10

N2 - Polyclonal antibodies against bovine milk lipoprotein lipase (LPL) were used to generate an enzyme-linked immunosorbent assay (ELISA) for rat LPL. The antibodies to LPL were affinity purified on bovine LPL columns and were shown to be specific for LPL by immunoprecipitation and enzyme inhibition. The solid-phase ELISA was sensitive from 1.0 to 20 ng/ml of LPL and paralleled enzyme activity. Denatured rat LPL showed the same LPL mass as undenatured samples, allowing LPL mass to be quantitated effectively in a variety of rat tissue extracts.

AB - Polyclonal antibodies against bovine milk lipoprotein lipase (LPL) were used to generate an enzyme-linked immunosorbent assay (ELISA) for rat LPL. The antibodies to LPL were affinity purified on bovine LPL columns and were shown to be specific for LPL by immunoprecipitation and enzyme inhibition. The solid-phase ELISA was sensitive from 1.0 to 20 ng/ml of LPL and paralleled enzyme activity. Denatured rat LPL showed the same LPL mass as undenatured samples, allowing LPL mass to be quantitated effectively in a variety of rat tissue extracts.

KW - ELISA

KW - bovine

KW - immunoassay

KW - lipoprotein lipase

KW - rat

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An enzyme-linked immunoassay for lipoprotein lipase

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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