Abstract
Polyclonal antibodies against bovine milk lipoprotein lipase (LPL) were used to generate an enzyme-linked immunosorbent assay (ELISA) for rat LPL. The antibodies to LPL were affinity purified on bovine LPL columns and were shown to be specific for LPL by immunoprecipitation and enzyme inhibition. The solid-phase ELISA was sensitive from 1.0 to 20 ng/ml of LPL and paralleled enzyme activity. Denatured rat LPL showed the same LPL mass as undenatured samples, allowing LPL mass to be quantitated effectively in a variety of rat tissue extracts.
| Original language | English |
|---|---|
| Pages (from-to) | 27-35 |
| Number of pages | 9 |
| Journal | Analytical Biochemistry |
| Volume | 166 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1987 |
Bibliographical note
Funding Information:This work was supported by the National Institutes of Health (HL2848 I, AM-HL 37085), the American Heart Association. Greater Los Angeles Affiliate (4921G 13). and the Veterans Administration. The authors also are indebted to Arlene S. Garfinkel for her help in assembling the manuscript.
Funding
This work was supported by the National Institutes of Health (HL2848 I, AM-HL 37085), the American Heart Association. Greater Los Angeles Affiliate (4921G 13). and the Veterans Administration. The authors also are indebted to Arlene S. Garfinkel for her help in assembling the manuscript.
| Funders | Funder number |
|---|---|
| National Institutes of Health (NIH) | HL2848 I, AM-HL 37085 |
| National Heart, Lung, and Blood Institute (NHLBI) | P01HL028481 |
| American Heart Association |
Keywords
- ELISA
- bovine
- immunoassay
- lipoprotein lipase
- rat
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology