An extracellular enzyme from Fusarium solani f. sp. phaseoli which catalyses hydration of the isoflavonoid phytoalexin, phaseollidin

Carol S. Turbek; David A. Smith; Christopher L. Schardl

doi:10.1016/0378-1097(92)90606-O

An extracellular enzyme from Fusarium solani f. sp. phaseoli which catalyses hydration of the isoflavonoid phytoalexin, phaseollidin

Carol S. Turbek, David A. Smith, Christopher L. Schardl

Plant Pathology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Among the antimicrobial phytoalexins produced by Phaseolus vulgaris (French bean) are the prenylated isoflavonoids kievitone and phaseollidin. Two enzyme activities, kievitone hydratase and phaseollidin hydratase, occur in culture filtrates of the bean pathogen, Fusarium solani f. sp. phaseoli, and catalyse similar hydration reactions on the dimethylallyl moieties of the phytoalexins. The enzymes nearly co-purified during hydroxyapatite chromatography followed by preparative native gel electrophoresis. Eluates from successive slices taken from the native gel were assayed for both activities. Although they were not completely separated in the native gel, the activity profiles indicated that the two activities were distinct. The K_m of phaseollidin hydratase for phaseollidin was approximately 7 μM.

Original language	English
Pages (from-to)	187-190
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	94
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1097(92)90606-O
State	Published - Jul 1 1992

Bibliographical note

Funding Information:
This work was supported by the United States Department of Agriculture, Competitive Re- search Grant 90-37262-5447. The investigation reported in this paper (No. 92-11-65) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with approval of the director.

Funding

This work was supported by the United States Department of Agriculture, Competitive Re- search Grant 90-37262-5447. The investigation reported in this paper (No. 92-11-65) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with approval of the director.

Funders	Funder number
U.S. Department of Agriculture	90-37262-5447

Keywords

Fusarium solani f. sp. phaseoli
Isoflavonoid phytoalexin
Kievitone hydratase
Phaseollidin hydratase
Phaseolus vulgaris

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1016/0378-1097(92)90606-O

Cite this

@article{e07f7bd43767406b85cd6d37dd2a637e,

title = "An extracellular enzyme from Fusarium solani f. sp. phaseoli which catalyses hydration of the isoflavonoid phytoalexin, phaseollidin",

abstract = "Among the antimicrobial phytoalexins produced by Phaseolus vulgaris (French bean) are the prenylated isoflavonoids kievitone and phaseollidin. Two enzyme activities, kievitone hydratase and phaseollidin hydratase, occur in culture filtrates of the bean pathogen, Fusarium solani f. sp. phaseoli, and catalyse similar hydration reactions on the dimethylallyl moieties of the phytoalexins. The enzymes nearly co-purified during hydroxyapatite chromatography followed by preparative native gel electrophoresis. Eluates from successive slices taken from the native gel were assayed for both activities. Although they were not completely separated in the native gel, the activity profiles indicated that the two activities were distinct. The Km of phaseollidin hydratase for phaseollidin was approximately 7 μM.",

keywords = "Fusarium solani f. sp. phaseoli, Isoflavonoid phytoalexin, Kievitone hydratase, Phaseollidin hydratase, Phaseolus vulgaris",

author = "Turbek, {Carol S.} and Smith, {David A.} and Schardl, {Christopher L.}",

note = "Funding Information: This work was supported by the United States Department of Agriculture, Competitive Re- search Grant 90-37262-5447. The investigation reported in this paper (No. 92-11-65) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with approval of the director.",

year = "1992",

month = jul,

day = "1",

doi = "10.1016/0378-1097(92)90606-O",

language = "English",

volume = "94",

pages = "187--190",

number = "1-2",

}

TY - JOUR

T1 - An extracellular enzyme from Fusarium solani f. sp. phaseoli which catalyses hydration of the isoflavonoid phytoalexin, phaseollidin

AU - Turbek, Carol S.

AU - Smith, David A.

AU - Schardl, Christopher L.

N1 - Funding Information: This work was supported by the United States Department of Agriculture, Competitive Re- search Grant 90-37262-5447. The investigation reported in this paper (No. 92-11-65) is in connection with a project of the Kentucky Agricultural Experiment Station and is published with approval of the director.

PY - 1992/7/1

Y1 - 1992/7/1

N2 - Among the antimicrobial phytoalexins produced by Phaseolus vulgaris (French bean) are the prenylated isoflavonoids kievitone and phaseollidin. Two enzyme activities, kievitone hydratase and phaseollidin hydratase, occur in culture filtrates of the bean pathogen, Fusarium solani f. sp. phaseoli, and catalyse similar hydration reactions on the dimethylallyl moieties of the phytoalexins. The enzymes nearly co-purified during hydroxyapatite chromatography followed by preparative native gel electrophoresis. Eluates from successive slices taken from the native gel were assayed for both activities. Although they were not completely separated in the native gel, the activity profiles indicated that the two activities were distinct. The Km of phaseollidin hydratase for phaseollidin was approximately 7 μM.

AB - Among the antimicrobial phytoalexins produced by Phaseolus vulgaris (French bean) are the prenylated isoflavonoids kievitone and phaseollidin. Two enzyme activities, kievitone hydratase and phaseollidin hydratase, occur in culture filtrates of the bean pathogen, Fusarium solani f. sp. phaseoli, and catalyse similar hydration reactions on the dimethylallyl moieties of the phytoalexins. The enzymes nearly co-purified during hydroxyapatite chromatography followed by preparative native gel electrophoresis. Eluates from successive slices taken from the native gel were assayed for both activities. Although they were not completely separated in the native gel, the activity profiles indicated that the two activities were distinct. The Km of phaseollidin hydratase for phaseollidin was approximately 7 μM.

KW - Fusarium solani f. sp. phaseoli

KW - Isoflavonoid phytoalexin

KW - Kievitone hydratase

KW - Phaseollidin hydratase

KW - Phaseolus vulgaris

UR - http://www.scopus.com/inward/record.url?scp=0026631313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026631313&partnerID=8YFLogxK

U2 - 10.1016/0378-1097(92)90606-O

DO - 10.1016/0378-1097(92)90606-O

M3 - Article

C2 - 1521768

AN - SCOPUS:0026631313

SN - 0378-1097

VL - 94

SP - 187

EP - 190

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1-2

ER -

An extracellular enzyme from Fusarium solani f. sp. phaseoli which catalyses hydration of the isoflavonoid phytoalexin, phaseollidin

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this