Abstract
Plants mount coordinated immune responses to defend themselves against pathogens. However, the cellular components required for plant immunity are not fully understood. The jasmonate-mimicking coronatine (COR) toxin produced by Pseudomonas syringae pv. tomato (Pst) DC3000 functions to overcome plant immunity. We previously isolated eight Arabidopsis (scord) mutants that exhibit increased susceptibility to a COR-deficient mutant of PstDC3000. Among them, the scord6 mutant exhibits defects both in stomatal closure response and in restricting bacterial multiplication inside the apoplast. However, the identity of SCORD6 remained elusive. In this study, we aim to identify the SCORD6 gene. We identified SCORD6 via next-generation sequencing and found it to be MURUS1 (MUR1), which is involved in the biosynthesis of GDP-l-fucose. Discovery of SCORD6 as MUR1 led to a series of experiments that revealed a multi-faceted role of l-fucose biosynthesis in stomatal and apoplastic defenses as well as in pattern-triggered immunity and effector-triggered immunity, including glycosylation of pattern-recognition receptors. Furthermore, compromised stomatal and/or apoplastic defenses were observed in mutants of several fucosyltransferases with specific substrates (e.g. O-glycan, N-glycan or the DELLA transcriptional repressors). Collectively, these results uncover a novel and broad role of l-fucose and protein fucosylation in plant immunity.
| Original language | English |
|---|---|
| Pages (from-to) | 981-994 |
| Number of pages | 14 |
| Journal | New Phytologist |
| Volume | 222 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 2019 |
Bibliographical note
Funding Information:TEM and SEM images were generated with the assistance of Dr Alicia Withrow and Carol Flegler (Center for Advanced Microscopy, MSU), respectively. The protocol for the stomatal closure assay was provided by Dr Yi-Ju Lu (MSU). qPCR primers for FRK1 were obtained from Dr Yuti Cheng (MSU). Phytohormone quantification was accomplished with the technical support of and the ABA-d6 internal standard from Drs Tony Schilmiller and Dan Jones (RTSF Mass Spectrometry and Metabolomics Core, MSU). This research was funded by US National Science Foundation (IOS-1557437 to BD and SYH), US Department of Agriculture – NIFA (2015-67017-23360 and 2017-67017-26180 to SYH), and US Department of Energy (the Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences, Office of Science; DE-FG02-91ER20021 for infrastructural support to SYH).
Funding Information:
TEM and SEM images were generated with the assistance of Dr Alicia Withrow and Carol Flegler (Center for Advanced Microscopy, MSU), respectively. The protocol for the stomatal closure assay was provided by Dr Yi-Ju Lu (MSU). qPCR primers for FRK1 were obtained from Dr Yuti Cheng (MSU). Phytohormone quantification was accomplished with the technical support of and the ABA-d6 internal standard from Drs Tony Schilmiller and Dan Jones (RTSF Mass Spectrometry and Metabolomics Core, MSU). This research was funded by US National Science Foundation (IOS-1557437 to BD and SYH), US Department of Agriculture ? NIFA (2015-67017-23360 and 2017-67017-26180 to SYH), and US Department of Energy (the Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences, Office of Science; DE-FG02-91ER20021 for infrastructural support to SYH).
Publisher Copyright:
© 2018 The Authors. New Phytologist © 2018 New Phytologist Trust
Keywords
- coronatine
- fucosylation
- jasmonate
- l-fucose
- plant immunity
- stomata
ASJC Scopus subject areas
- Physiology
- Plant Science