An Improved Filtration Procedure for Measuring Opiate Receptors in Small Regions of Rat Brain

M. T. Bardo; R. K. Bhatnagar; G. F. Gebhart

doi:10.1111/j.1471-4159.1982.tb08015.x

An Improved Filtration Procedure for Measuring Opiate Receptors in Small Regions of Rat Brain

M. T. Bardo, R. K. Bhatnagar, G. F. Gebhart

Research output: Contribution to journal › Article › peer-review

Abstract

Abstract: A modified filtration method for in vitro receptor binding was used to determine specific binding of [³H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 μg of protein per assay tube. Large differences in [³H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.

Original language	English
Pages (from-to)	1751-1754
Number of pages	4
Journal	Journal of Neurochemistry
Volume	39
Issue number	6
DOIs	https://doi.org/10.1111/j.1471-4159.1982.tb08015.x
State	Published - Dec 1982

Keywords

Brain nuclei
Naloxone
Opiate receptor

ASJC Scopus subject areas

Biochemistry
Cellular and Molecular Neuroscience

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10.1111/j.1471-4159.1982.tb08015.x

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N2 - Abstract: A modified filtration method for in vitro receptor binding was used to determine specific binding of [3H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 μg of protein per assay tube. Large differences in [3H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.

AB - Abstract: A modified filtration method for in vitro receptor binding was used to determine specific binding of [3H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 μg of protein per assay tube. Large differences in [3H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.

KW - Brain nuclei

KW - Naloxone

KW - Opiate receptor

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An Improved Filtration Procedure for Measuring Opiate Receptors in Small Regions of Rat Brain

Abstract

Keywords

ASJC Scopus subject areas

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