An increase in S-glutathionylated proteins in the Alzheimer's disease inferior parietal lobule, a proteomics approach

Shelley F. Newman, Rukhsana Sultana, Marzia Perluigi, Rafella Coccia, Jian Cai, William M. Pierce, Jon B. Klein, Delano M. Turner, D. Allan Butterfield

Research output: Contribution to journalArticlepeer-review

152 Scopus citations

Abstract

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by neurofibrillary tangles, senile plaques, and loss of synapses. Many studies support the notion that oxidative stress plays an important role in AD pathogenesis. Previous studies from our laboratory employed redox proteomics to identify oxidatively modified proteins in the AD inferior parietal lobule (IPL) and hippocampus. The proteins were consistent with biochemical or pathological alterations in AD and have been central to further investigations of the disease. The present study focused on the identification of specific targets of protein S-glutathionylation in AD and control IPL by using a redox proteomics approach. For AD IPL, we identified deoxyhemoglobin, α-crystallin B, glyceraldehyde phosphate dehydrogenase (GAPDH), and α-enolase as significantly S-glutathionylated relative to these brain proteins in control IPL. GAPDH and α-enolase were also shown to have reduced activity in the AD IPL. This study demonstrates that specific proteins are sensitive to S-glutathionylation, which most likely is due to their sensitivity to cysteine oxidation initiated by the increase in oxidative stress in the AD brain.

Original languageEnglish
Pages (from-to)1506-1514
Number of pages9
JournalJournal of Neuroscience Research
Volume85
Issue number7
DOIs
StatePublished - May 15 2007

Keywords

  • Alzheimer's disease
  • GAPDH
  • Glutathione
  • Redox proteomics
  • α-enolase

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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