TY - JOUR
T1 - An instrument for the measurement of rapid reaction kinetics
AU - McCarthy, John J.
AU - Henry, Shawn O.
AU - Luckin, Kristen A.
AU - Cholewinski, Norman D.
AU - Klug, Gary A.
PY - 1994/9
Y1 - 1994/9
N2 - Two versions of an inexpensive mixing device capable of measuring reaction kinetics with millisecond resolution are described. The instruments deliver reactants via microsyringes controlled by nitrogen pressure at time intervals that are either mechanically or software controlled. In addition, an optical system for calibration of the time required to deliver the reactants as well as the intervals between syringe firing is given. Data illustrating the effect of variations in volume and syringe size on the accuracy and reproducibility of these time measurements as well as the volume of fluid delivered are presented. Examples of kinetic measurements of several reactions illustrating the capabilities of the instrument are also presented.
AB - Two versions of an inexpensive mixing device capable of measuring reaction kinetics with millisecond resolution are described. The instruments deliver reactants via microsyringes controlled by nitrogen pressure at time intervals that are either mechanically or software controlled. In addition, an optical system for calibration of the time required to deliver the reactants as well as the intervals between syringe firing is given. Data illustrating the effect of variations in volume and syringe size on the accuracy and reproducibility of these time measurements as well as the volume of fluid delivered are presented. Examples of kinetic measurements of several reactions illustrating the capabilities of the instrument are also presented.
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U2 - 10.1006/abio.1994.1409
DO - 10.1006/abio.1994.1409
M3 - Article
C2 - 7810864
AN - SCOPUS:0028136905
SN - 0003-2697
VL - 221
SP - 250
EP - 265
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -