An instrument for the measurement of rapid reaction kinetics

John J. McCarthy; Shawn O. Henry; Kristen A. Luckin; Norman D. Cholewinski; Gary A. Klug

doi:10.1006/abio.1994.1409

An instrument for the measurement of rapid reaction kinetics

John J. McCarthy, Shawn O. Henry, Kristen A. Luckin, Norman D. Cholewinski, Gary A. Klug

Physiology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Two versions of an inexpensive mixing device capable of measuring reaction kinetics with millisecond resolution are described. The instruments deliver reactants via microsyringes controlled by nitrogen pressure at time intervals that are either mechanically or software controlled. In addition, an optical system for calibration of the time required to deliver the reactants as well as the intervals between syringe firing is given. Data illustrating the effect of variations in volume and syringe size on the accuracy and reproducibility of these time measurements as well as the volume of fluid delivered are presented. Examples of kinetic measurements of several reactions illustrating the capabilities of the instrument are also presented.

Original language	English
Pages (from-to)	250-265
Number of pages	16
Journal	Analytical Biochemistry
Volume	221
Issue number	2
DOIs	https://doi.org/10.1006/abio.1994.1409
State	Published - Sep 1994

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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An instrument for the measurement of rapid reaction kinetics

Abstract

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