An integrated approach for automating validation of extracted ion chromatographic peaks

William D. Nelson; Kert Viele; Bert C. Lynn

doi:10.1093/bioinformatics/btn385

An integrated approach for automating validation of extracted ion chromatographic peaks

William D. Nelson, Kert Viele, Bert C. Lynn

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Accurate determination of extracted ion chromatographic peak areas in isotope-labeled quantitative proteomics is difficult to automate. Manual validation of identified peaks is typically required. We have integrated a peak confidence scoring algorithm into existing tools which are compatible with analysis pipelines based on the standards from the Institute for Systems Biology. This algorithm automatically excludes incorrectly identified peaks, improving the accuracy of the final protein expression ratio calculation.

Original language	English
Pages (from-to)	2103-2104
Number of pages	2
Journal	Bioinformatics
Volume	24
Issue number	18
DOIs	https://doi.org/10.1093/bioinformatics/btn385
State	Published - Sep 2008

Bibliographical note

Funding Information:
Funding: This research was supported by NCRR(NIH) Grant P 20 RR16481 and Kentucky EPSCoR award CRDG-006-06.

ASJC Scopus subject areas

Statistics and Probability
Biochemistry
Molecular Biology
Computer Science Applications
Computational Theory and Mathematics
Computational Mathematics

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10.1093/bioinformatics/btn385

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abstract = "Accurate determination of extracted ion chromatographic peak areas in isotope-labeled quantitative proteomics is difficult to automate. Manual validation of identified peaks is typically required. We have integrated a peak confidence scoring algorithm into existing tools which are compatible with analysis pipelines based on the standards from the Institute for Systems Biology. This algorithm automatically excludes incorrectly identified peaks, improving the accuracy of the final protein expression ratio calculation.",

author = "Nelson, {William D.} and Kert Viele and Lynn, {Bert C.}",

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AU - Viele, Kert

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N2 - Accurate determination of extracted ion chromatographic peak areas in isotope-labeled quantitative proteomics is difficult to automate. Manual validation of identified peaks is typically required. We have integrated a peak confidence scoring algorithm into existing tools which are compatible with analysis pipelines based on the standards from the Institute for Systems Biology. This algorithm automatically excludes incorrectly identified peaks, improving the accuracy of the final protein expression ratio calculation.

AB - Accurate determination of extracted ion chromatographic peak areas in isotope-labeled quantitative proteomics is difficult to automate. Manual validation of identified peaks is typically required. We have integrated a peak confidence scoring algorithm into existing tools which are compatible with analysis pipelines based on the standards from the Institute for Systems Biology. This algorithm automatically excludes incorrectly identified peaks, improving the accuracy of the final protein expression ratio calculation.

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An integrated approach for automating validation of extracted ion chromatographic peaks

Abstract

Bibliographical note

ASJC Scopus subject areas

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