Abstract
The genome of Potato yellow dwarf virus (PYDV; Nucleorhabdovirus type species) was determined to be 12,875 nucleotides (nt). The antigenome is organized into seven open reading frames (ORFs) ordered 3'-N-X-P-Y-M-G-L-5', which likely encode the nucleocapsid, phospho, movement, matrix, glyco and RNA-dependent RNA polymerase proteins, respectively, except for X, which is of unknown function. The ORFs are flanked by a 3' leader RNA of 149 nt and a 5' trailer RNA of 97 nt, and are separated by conserved intergenic junctions. Phylogenetic analyses indicated that PYDV is closely related to other leafhopper-transmitted rhabdoviruses. Functional protein assays were used to determine the subcellular localization of PYDV proteins. Surprisingly, the M protein was able to induce the intranuclear accumulation of the inner nuclear membrane in the absence of any other viral protein. Finally, bimolecular fluorescence complementation was used to generate the most comprehensive protein interaction map for a plant-adapted rhabdovirus to date.
| Original language | English |
|---|---|
| Pages (from-to) | 61-71 |
| Number of pages | 11 |
| Journal | Virology |
| Volume | 402 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 2010 |
Bibliographical note
Funding Information:We wish to express our sincere appreciation to Hei-ti Hsu and Andrew Jackson for providing stocks of PYDV and antisera. We thank Vitaly Citovsky for providing pNIAc and Byoung-Eun Min for converting it to Gateway compatibility. We are grateful to Ralf Dietzgen for providing a critical review of the manuscript prior to submission. We also thank the Kentucky Tobacco Research and Development Center, the National Institutes of Health and the National Science Foundation for grant support to MG.
Keywords
- BiFC
- Confocal
- FRAP
- GFP
- Interactome
- Localization
- Nicotiana benthamiana
- Nuclear localization
- Rhabdovirus
- TagRFP
ASJC Scopus subject areas
- Virology