TY - JOUR
T1 - An invertebrate model in examining the effect of acute ferric iron exposure on proprioceptive neurons
AU - Wagers, Mikaela L.
AU - Starks, Ashley
AU - Abul-Khoudoud, Maya O.
AU - Ahmed, Sufia M.
AU - Alhamdani, Abraham W.
AU - Ashley, Clair
AU - Bidros, Patrick C.
AU - Bledsoe, Constance O.
AU - Bolton, Kayli E.
AU - Capili, Jerone G.
AU - Henning, Jamie N.
AU - Ison, Bethany J.
AU - Moon, Madison
AU - Phe, Panhavuth
AU - Stonecipher, Samuel B.
AU - Taylor, Isabelle N.
AU - Turner, Logan T.
AU - West, Aaron K.
AU - Cooper, Robin L.
N1 - Funding Information:
Department of Biology, University of Kentucky. Chellgren Endowed Funding (R.L.C.). College of Arts and Sciences summer fellowship (M.L.W.).
Publisher Copyright:
© 2023
PY - 2023/4
Y1 - 2023/4
N2 - Iron is an essential element for plant and animal life and is found in soil, fresh waters and marine waters. The Fe3+ ion is a vital prosthetic group and cofactor to mitochondrial electron transport complexes and numerous proteins involved in normal functioning. Despite its importance to life-sustaining processes, overexposure results in toxicity. For example, ferric iron (Fe3+) accumulation in the mammalian central nervous system is associated with various neurological disorders. Although current literature addresses the long-term effects of Fe3+ overload, fewer studies exist examining the effects of acute exposure. Using the blue crab (Callinectes sapidus), we investigate the effects of acute Fe3+ overload on proprioception within the propodite-dactylopodite (PD) nerve. For proprioceptive studies, 10- and 20-mM ferric chloride and ferric ammonium citrate solutions were used at 5- and 20- min exposure times. Exposure to 20 mM concentrations of ferric chloride and ferric ammonium citrate reduced excitability in proprioceptive neurons. Thus, Fe3+ likely blocks stretch-activated channels or voltage-gated Na+ channels. The depressive effects of Fe3+ are partly reversible following saline washout, indicating cells are not acutely damaged. Gadolinium (GdCl3, 1 and 10 mM) was used to examine the effects of an additional trivalent ion comparator. Gd3+ depressed PD nerve compound action potential amplitude while increasing the compound action potential duration. This study is relevant in demonstrating the dose-dependent effects of acute Fe3+ and Gd3+ exposure on proprioception and provides a model system to further investigate the mechanisms by which metals act on the nervous system.
AB - Iron is an essential element for plant and animal life and is found in soil, fresh waters and marine waters. The Fe3+ ion is a vital prosthetic group and cofactor to mitochondrial electron transport complexes and numerous proteins involved in normal functioning. Despite its importance to life-sustaining processes, overexposure results in toxicity. For example, ferric iron (Fe3+) accumulation in the mammalian central nervous system is associated with various neurological disorders. Although current literature addresses the long-term effects of Fe3+ overload, fewer studies exist examining the effects of acute exposure. Using the blue crab (Callinectes sapidus), we investigate the effects of acute Fe3+ overload on proprioception within the propodite-dactylopodite (PD) nerve. For proprioceptive studies, 10- and 20-mM ferric chloride and ferric ammonium citrate solutions were used at 5- and 20- min exposure times. Exposure to 20 mM concentrations of ferric chloride and ferric ammonium citrate reduced excitability in proprioceptive neurons. Thus, Fe3+ likely blocks stretch-activated channels or voltage-gated Na+ channels. The depressive effects of Fe3+ are partly reversible following saline washout, indicating cells are not acutely damaged. Gadolinium (GdCl3, 1 and 10 mM) was used to examine the effects of an additional trivalent ion comparator. Gd3+ depressed PD nerve compound action potential amplitude while increasing the compound action potential duration. This study is relevant in demonstrating the dose-dependent effects of acute Fe3+ and Gd3+ exposure on proprioception and provides a model system to further investigate the mechanisms by which metals act on the nervous system.
KW - Crustacean
KW - Gadolinium
KW - Iron
KW - Neurophysiology
KW - Sensory
KW - Stretch activated channels
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U2 - 10.1016/j.cbpc.2023.109558
DO - 10.1016/j.cbpc.2023.109558
M3 - Article
C2 - 36717044
AN - SCOPUS:85149154481
SN - 1532-0456
VL - 266
JO - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
M1 - 109558
ER -