An Ozonolysis Based Method and Applications for the Non-Lethal Modification of Insect Cuticular Hydrocarbons

Benjamin Savage; Zinan Wang; Henry Chung; Susan Masten; Matthew Grieshop

doi:10.1007/s10886-021-01285-8

An Ozonolysis Based Method and Applications for the Non-Lethal Modification of Insect Cuticular Hydrocarbons

Benjamin Savage
, Zinan Wang
, Henry Chung
, Susan Masten
, Matthew Grieshop

Entomology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Cuticular hydrocarbons (CHCs) are important, multi-function components of the insect epicuticle. In Drosophila spp., CHCs provide protection from desiccation and serve as semiochemicals for both intra- and interspecific communication. We developed a non-lethal method for the modification of Drosophila CHCs profiles through the exposure of live insects to a high dose of ozone gas (~ 45,000 ppm). Drosophila suzukii that were treated with ozone showed a 1.63–3.10 fold reduction in unsaturated hydrocarbons with these CHCs shown to regenerate over 108 h. Changes in CHCs were correlated with significantly reduced desiccation resistance in both male and female D. suzukii at one h after ozone treatment. Interestingly, individuals treated with ozone showed increased desiccation resistance in comparison to controls at 108 h after ozone treatment. The methodology reported in this paper provides a novel approach to investigate the biosynthesis and functions of CHCs during the lifespan of an insect.

Original language	English
Pages (from-to)	628-641
Number of pages	14
Journal	Journal of Chemical Ecology
Volume	47
Issue number	7
DOIs	https://doi.org/10.1007/s10886-021-01285-8
State	Published - Jul 2021

Bibliographical note

Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

Funding

Experiments were made possible with the help of Allison Fischer, Charlotte Schuttler, Elizabeth Szcepanski, Kyle Akred, Keith Koonter, Juan Huang, Ph.D., and Phil Fanning, Ph.D. Experiments were performed at the Organic Pest Management lab at Michigan State University directed by Matthew Grieshop, Ph.D.

Funders
Michigan State University

Keywords

Cuticular hydrocarbons
Desiccation resistance
Drosophila suzukii
Modification
Ozone

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Biochemistry

Access to Document

10.1007/s10886-021-01285-8

Cite this

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title = "An Ozonolysis Based Method and Applications for the Non-Lethal Modification of Insect Cuticular Hydrocarbons",

abstract = "Cuticular hydrocarbons (CHCs) are important, multi-function components of the insect epicuticle. In Drosophila spp., CHCs provide protection from desiccation and serve as semiochemicals for both intra- and interspecific communication. We developed a non-lethal method for the modification of Drosophila CHCs profiles through the exposure of live insects to a high dose of ozone gas (\textasciitilde{} 45,000 ppm). Drosophila suzukii that were treated with ozone showed a 1.63{\textendash}3.10 fold reduction in unsaturated hydrocarbons with these CHCs shown to regenerate over 108 h. Changes in CHCs were correlated with significantly reduced desiccation resistance in both male and female D. suzukii at one h after ozone treatment. Interestingly, individuals treated with ozone showed increased desiccation resistance in comparison to controls at 108 h after ozone treatment. The methodology reported in this paper provides a novel approach to investigate the biosynthesis and functions of CHCs during the lifespan of an insect.",

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AU - Savage, Benjamin

AU - Wang, Zinan

AU - Chung, Henry

AU - Masten, Susan

AU - Grieshop, Matthew

PY - 2021/7

Y1 - 2021/7

N2 - Cuticular hydrocarbons (CHCs) are important, multi-function components of the insect epicuticle. In Drosophila spp., CHCs provide protection from desiccation and serve as semiochemicals for both intra- and interspecific communication. We developed a non-lethal method for the modification of Drosophila CHCs profiles through the exposure of live insects to a high dose of ozone gas (~ 45,000 ppm). Drosophila suzukii that were treated with ozone showed a 1.63–3.10 fold reduction in unsaturated hydrocarbons with these CHCs shown to regenerate over 108 h. Changes in CHCs were correlated with significantly reduced desiccation resistance in both male and female D. suzukii at one h after ozone treatment. Interestingly, individuals treated with ozone showed increased desiccation resistance in comparison to controls at 108 h after ozone treatment. The methodology reported in this paper provides a novel approach to investigate the biosynthesis and functions of CHCs during the lifespan of an insect.

AB - Cuticular hydrocarbons (CHCs) are important, multi-function components of the insect epicuticle. In Drosophila spp., CHCs provide protection from desiccation and serve as semiochemicals for both intra- and interspecific communication. We developed a non-lethal method for the modification of Drosophila CHCs profiles through the exposure of live insects to a high dose of ozone gas (~ 45,000 ppm). Drosophila suzukii that were treated with ozone showed a 1.63–3.10 fold reduction in unsaturated hydrocarbons with these CHCs shown to regenerate over 108 h. Changes in CHCs were correlated with significantly reduced desiccation resistance in both male and female D. suzukii at one h after ozone treatment. Interestingly, individuals treated with ozone showed increased desiccation resistance in comparison to controls at 108 h after ozone treatment. The methodology reported in this paper provides a novel approach to investigate the biosynthesis and functions of CHCs during the lifespan of an insect.

KW - Cuticular hydrocarbons

KW - Desiccation resistance

KW - Drosophila suzukii

KW - Modification

KW - Ozone

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IS - 7

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An Ozonolysis Based Method and Applications for the Non-Lethal Modification of Insect Cuticular Hydrocarbons

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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