Analysis and modification of ergot alkaloid profiles in fungi

Daniel G. Panaccione; Katy L. Ryan; Christopher L. Schardl; Simona Florea

doi:10.1016/B978-0-12-394290-6.00012-4

Analysis and modification of ergot alkaloid profiles in fungi

Daniel G. Panaccione, Katy L. Ryan, Christopher L. Schardl, Simona Florea

Plant Pathology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

43 Scopus citations

Abstract

The ergot alkaloids are a family of secondary metabolites produced by a phylogenetically discontinuous group of fungi. Various members of the family are important in agriculture, where they accumulate in grain crops or forage grasses and adversely affect humans or animals who consume them. Other ergot alkaloids have been used clinically to treat a variety of diseases. Because of their significance in agriculture and medicine, the ability to detect and quantify these alkaloids from a variety of substrates is important. The primary analytical approach for these purposes has been high performance liquid chromatography. The ability to manipulate ergot alkaloid production in fungi, by transformation-mediated approaches, has been useful for studies on the biosynthesis of these alkaloids and may have practical application in agriculture and medicine. Such modifications have been informed by comparative genomic approaches, which have provided information on the gene clusters associated with ergot alkaloid biosynthesis.

Original language	English
Title of host publication	Methods in Enzymology
Pages	267-290
Number of pages	24
DOIs	https://doi.org/10.1016/B978-0-12-394290-6.00012-4
State	Published - 2012

Publication series

Name	Methods in Enzymology
Volume	515
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

Bibliographical note

Funding Information:
We gratefully acknowledge funding from the U.S. Department of Agriculture National Institute of Food and Agriculture (2008-35318-04549 and 2012-67013-19384). This chapter is published with permission of the West Virginia Agriculture and Forestry Experiment Station as scientific article number 3125.

Keywords

Clavines
Ergot alkaloids
Gene clusters
Lysergyls
Mycotoxins
Secondary metabolism

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/B978-0-12-394290-6.00012-4

Cite this

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abstract = "The ergot alkaloids are a family of secondary metabolites produced by a phylogenetically discontinuous group of fungi. Various members of the family are important in agriculture, where they accumulate in grain crops or forage grasses and adversely affect humans or animals who consume them. Other ergot alkaloids have been used clinically to treat a variety of diseases. Because of their significance in agriculture and medicine, the ability to detect and quantify these alkaloids from a variety of substrates is important. The primary analytical approach for these purposes has been high performance liquid chromatography. The ability to manipulate ergot alkaloid production in fungi, by transformation-mediated approaches, has been useful for studies on the biosynthesis of these alkaloids and may have practical application in agriculture and medicine. Such modifications have been informed by comparative genomic approaches, which have provided information on the gene clusters associated with ergot alkaloid biosynthesis.",

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note = "Funding Information: We gratefully acknowledge funding from the U.S. Department of Agriculture National Institute of Food and Agriculture (2008-35318-04549 and 2012-67013-19384). This chapter is published with permission of the West Virginia Agriculture and Forestry Experiment Station as scientific article number 3125.",

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AU - Ryan, Katy L.

AU - Schardl, Christopher L.

AU - Florea, Simona

N1 - Funding Information: We gratefully acknowledge funding from the U.S. Department of Agriculture National Institute of Food and Agriculture (2008-35318-04549 and 2012-67013-19384). This chapter is published with permission of the West Virginia Agriculture and Forestry Experiment Station as scientific article number 3125.

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AB - The ergot alkaloids are a family of secondary metabolites produced by a phylogenetically discontinuous group of fungi. Various members of the family are important in agriculture, where they accumulate in grain crops or forage grasses and adversely affect humans or animals who consume them. Other ergot alkaloids have been used clinically to treat a variety of diseases. Because of their significance in agriculture and medicine, the ability to detect and quantify these alkaloids from a variety of substrates is important. The primary analytical approach for these purposes has been high performance liquid chromatography. The ability to manipulate ergot alkaloid production in fungi, by transformation-mediated approaches, has been useful for studies on the biosynthesis of these alkaloids and may have practical application in agriculture and medicine. Such modifications have been informed by comparative genomic approaches, which have provided information on the gene clusters associated with ergot alkaloid biosynthesis.

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KW - Gene clusters

KW - Lysergyls

KW - Mycotoxins

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Analysis and modification of ergot alkaloid profiles in fungi

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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