Analysis of multiple molecular changes in human endocrine tumours

I. Arany, P. Rady, B. M. Evers, S. K. Tyring, C. M. Townsend

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


To define the molecular changes occurring in endocrine tumours, we have analysed three human endocrine tumours established in our laboratory: BON, a functioning carcinoid tumour from the pancreas; SIM, a nonfunctioning carcinoid of the ileum; and STAN, a pheochromocytoma. A homozygous point mutation of the N-ras gene was identified at codon 61 in BON cells in conjunction with overexpression of N-ras mRNA and protein. BON cells also exhibited increased expression of c-myc and cdc2 kinase mRNA and protein; TGF-β1, p53 and retinoblastoma (RB) mRNA and protein levels were decreased. In addition, increased expression of the mdm2 oncogene and both the truncated and the wild-type RB protein were noted in BON. SIM cells exhibited moderately increased N-ras and c-myc mRNA levels along with decreased levels of RB mRNA and protein. Similar to BON and SIM, analysis of STAN showed increased N-ras and c-myc levels. Our data show multiple molecular changes in the three human endocrine tumours with the BON cell line exhibiting the most dramatic changes. Furthermore, our data suggest the existence of different molecular pathways in the pathogenesis of endocrine tumours. These cell lines will provide unique in vitro models to further analyse the significance of these molecular alterations.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalSurgical Oncology
Issue number3
StatePublished - Jun 1994

Bibliographical note

Funding Information:
This study was supported by grants from the National institute of Allergy and Infectious Disease (R01266896, NIH NCRR GCRC and DHHS MO1 RR00073), the National Institutes of Health (R29 AG10885) and by a grant from the American Cancer Society (CB-571).


  • cytokines
  • endocrine tumours
  • oncogenes
  • tumour suppressor genes

ASJC Scopus subject areas

  • Surgery
  • Oncology


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