Analysis of nitric oxide activity in prevention of reperfusion injury

James Emmett Lovett, Betsy F. Fink, Andrew Bernard, Juan Ochoa

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


This project was designed to determine the role of nitric oxide (NO) in the prevention of ischemia-reperfusion injury. Inferiorly based rectus abdominis muscle flaps were elevated in pigs and subjected to 6 hours of ischemia followed by 4 hours of reperfusion. Group I animals received a bolus of L-arginine before reperfusion, and a continuous infusion once flow was restored. Group II animals served as controls and received an equal volume of saline as a bolus and subsequent continuous infusion. Microdialysis was used to measure tissue NO levels, and these were correlated with muscle survival determined by vital staining with nitroblue tetrazolium. The results demonstrated a significant increase in tissue NO levels in L-arginine-supplemented animals (p < 0.05), which in turn correlated with a significant increase in muscle survival (p = 0.0051). These results suggest that administration of supplemental L-arginine to ischemic skeletal muscle during reperfusion results in increased NO production and decreased tissue damage.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalAnnals of Plastic Surgery
Issue number3
StatePublished - 2001

ASJC Scopus subject areas

  • Surgery


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