Analysis of various physiological salines for heart rate, CNS function, and synaptic transmission at neuromuscular junctions in Drosophila melanogaster larvae

Clara de Castro, Josh Titlow, Zana R. Majeed, Robin L. Cooper

Research output: Contribution to journalArticlepeer-review

27 Citations (SciVal)

Abstract

Drosophila serves as a playground for examining the effects of genetic mutations on development, physiological function and behavior. Many physiological measures that address the effects of mutations require semi-intact or cultured preparations. To obtain consistent physiological recordings, cellular function needs to remain viable. Numerous physiological salines have been developed for fly preparations, with emphasis on nervous system viability. The commonly used saline drifts in pH and will cause an alteration in the heart rate. We identify a saline that maintains a stable pH and physiological function in the larval heart, skeletal neuromuscular junction, and ventral nerve cord preparations. Using these common assays, we screened various pH buffers of differing concentrations to identify optimum conditions. Buffers at 25 mM produce a stable heart rate with minimal variation in pH. Excitatory junction potentials evoked directly on larval muscles or through sensory-CNS-motor circuits were unaffected by at buffers at 25 mM. The salines examined did not impede the modulatory effect of serotonin on heart rate or neural activity. Together, our results indicate that the higher buffer concentrations needed to stabilize pH in HL3 hemolymph-like saline do not interfere with the acute function of neurons or cardiac myocytes.

Original languageEnglish
Pages (from-to)83-92
Number of pages10
JournalJournal of Comparative Physiology A
Volume200
Issue number1
DOIs
StatePublished - Jan 2014

Bibliographical note

Funding Information:
Acknowledgments This work was funded by the Higher Committee for education Development (HCeD) in Iraq (ZrM) and personal funds (rlC). We thank the former high school students from lexington, Kentucky Ms. Kylah rymond and Ms. Valarie Sarge for preliminary studies in modifying the concentrations in amino acids and Ca2+ in the Hl3 saline which lead to this current project.

Keywords

  • Heart
  • Physiology
  • Saline
  • Synaptic transmission

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Physiology
  • Animal Science and Zoology
  • Behavioral Neuroscience

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