TY - JOUR
T1 - Angiotensin-converting enzyme 2 deficiency in whole body or bone marrow-derived cells increases atherosclerosis in low-density lipoprotein receptor -/- mice
AU - Thatcher, Sean E.
AU - Zhang, Xuan
AU - Howatt, Deborah A.
AU - Lu, Hong
AU - Gurley, Susan B.
AU - Daugherty, Alan
AU - Cassis, Lisa A.
PY - 2011/4
Y1 - 2011/4
N2 - Objective- The renin-angiotensin system contributes to atherosclerotic lesion formation. Angiotensin-converting enzyme 2 (ACE2) catabolizes angiotensin II (Ang II) to angiotensin 1-7 (Ang-(1-7)) to limit effects of the renin-angiotensin system. The purpose of this study was to define the role of ACE2 in atherosclerosis. Methods and Results- Male Ace2 -/y mice in an low-density lipoprotein receptor-deficient background were fed a high-fat diet for 3 months. ACE2 deficiency increased atherosclerotic area (Ace2 +/y, 17±1; Ace2 -/y, 23±2 mm 2, P<0.002). This increase was blunted by losartan. To determine whether leukocytic ACE2 influenced atherosclerosis, irradiated low-density lipoprotein receptor-deficient male mice were repopulated with bone marrow-derived cells from Ace2 +/y or Ace2 -/y mice and fed a high-fat diet for 3 months. ACE2 deficiency in bone marrow-derived cells increased atherosclerotic area (Ace2 +/y, 1.6±0.3; Ace2 -/y, 2.8±0.3 mm 2; P<0.05). Macrophages from Ace2 -/y mice exhibited increased Ang II secretion and elevated expression of inflammatory cytokines. Conditioned media from mouse peritoneal macrophages of Ace2 +/y mice increased monocyte adhesion to human umbilical vein endothelial cells. Incubation of human umbilical vein endothelial cells with Ang II promoted monocyte adhesion, which was blocked by Ang-(1-7). Coinfusion of Ang-(1-7) with Ang II reduced atherosclerosis. Conclusion- These results demonstrate that ACE2 deficiency in bone marrow-derived cells promotes atherosclerosis through regulation of Ang II/Ang-(1-7) peptides.
AB - Objective- The renin-angiotensin system contributes to atherosclerotic lesion formation. Angiotensin-converting enzyme 2 (ACE2) catabolizes angiotensin II (Ang II) to angiotensin 1-7 (Ang-(1-7)) to limit effects of the renin-angiotensin system. The purpose of this study was to define the role of ACE2 in atherosclerosis. Methods and Results- Male Ace2 -/y mice in an low-density lipoprotein receptor-deficient background were fed a high-fat diet for 3 months. ACE2 deficiency increased atherosclerotic area (Ace2 +/y, 17±1; Ace2 -/y, 23±2 mm 2, P<0.002). This increase was blunted by losartan. To determine whether leukocytic ACE2 influenced atherosclerosis, irradiated low-density lipoprotein receptor-deficient male mice were repopulated with bone marrow-derived cells from Ace2 +/y or Ace2 -/y mice and fed a high-fat diet for 3 months. ACE2 deficiency in bone marrow-derived cells increased atherosclerotic area (Ace2 +/y, 1.6±0.3; Ace2 -/y, 2.8±0.3 mm 2; P<0.05). Macrophages from Ace2 -/y mice exhibited increased Ang II secretion and elevated expression of inflammatory cytokines. Conditioned media from mouse peritoneal macrophages of Ace2 +/y mice increased monocyte adhesion to human umbilical vein endothelial cells. Incubation of human umbilical vein endothelial cells with Ang II promoted monocyte adhesion, which was blocked by Ang-(1-7). Coinfusion of Ang-(1-7) with Ang II reduced atherosclerosis. Conclusion- These results demonstrate that ACE2 deficiency in bone marrow-derived cells promotes atherosclerosis through regulation of Ang II/Ang-(1-7) peptides.
KW - ACE2
KW - angiotensin II
KW - atherosclerosis
KW - leukocytes
KW - macrophages
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U2 - 10.1161/ATVBAHA.110.221614
DO - 10.1161/ATVBAHA.110.221614
M3 - Article
C2 - 21252069
AN - SCOPUS:79953742944
SN - 1079-5642
VL - 31
SP - 758
EP - 765
JO - Arteriosclerosis, Thrombosis, and Vascular Biology
JF - Arteriosclerosis, Thrombosis, and Vascular Biology
IS - 4
ER -