Angiotensinogen in hepatocytes contributes to Western diet-induced liver steatosis

Nonalcoholic fatty liver disease (NAFLD) is considered as a liver manifestation of metabolic disorders. Previous studies indicate that the renin-angiotensin system (RAS) plays a complex role in NAFLD. As the only precursor of the RAS, decreased angiotensinogen (AGT) profoundly impacts RAS bioactivity. Here, we investigated the role of hepatocyte-derived AGT in liver steatosis. AGT floxed mice (hepAGT^+/+) and hepatocyte-specific AGT-deficient mice (hepAGT^-/-) were fed a Western diet and a normal laboratory diet for 12 weeks, respectively. Compared with hep- AGT^+/+ mice, Western diet-fed hepAGT^-/- mice gained less body weight with improved insulin sensitivity. The attenuated severity of liver steatosis in hepAGT^-/- mice was evidenced by histologic changes and reduced intrahepatic triglycerides. The abundance of SREBP1 and its downstream molecules, acetyl-CoA carboxylase and FASN, was suppressed in hepAGT^-/- mice. Furthermore, serum derived from hepAGT^+/+ mice stimulated hepatocyte SREBP1 expression, which could be diminished by protein kinase B (Akt)/mammalian target of rapamycin (mTOR) inhibition in vitro. Administration of losartan did not affect diet-induced body weight gain, liver steatosis severity, and hepatic p-Akt, p-mTOR, and SREBP1 protein abundance in hepAGT^+/+ mice. These data suggest that attenuation of Western dietinduced liver steatosis in hepAGT^-/- mice is associated with the alternation of the Akt/mTOR/SREBP-1c pathway.