Antagonistic actions of juvenile hormone and 20-hydroxyecdysone within the ring gland determine developmental transitions in Drosophila

Suning Liu, Kang Li, Yue Gao, Xi Liu, Weiting Chen, Wei Ge, Qili Feng, Subba R. Palli, Sheng Li

Research output: Contribution to journalArticlepeer-review

119 Scopus citations

Abstract

In both vertebrates and insects, developmental transition from the juvenile stage to adulthood is regulated by steroid hormones. In insects, the steroid hormone, 20-hydroxyecdysone (20E), elicits metamorphosis, thus promoting this transition, while the sesquiterpenoid juvenile hormone (JH) antagonizes 20E signaling to prevent precocious metamorphosis during the larval stages. However, not much is known about the mechanisms involved in crosstalk between these two hormones. In this study, we discovered that in the ring gland (RG) of Drosophila larvae, JH and 20E control each other’s biosynthesis. JH induces expression of a Krüppel-like transcription factor gene Kr-h1 in the prothoracic gland (PG), a portion of the RG that produces the 20E precursor ecdysone. By reducing both steroidogenesis autoregulation and PG size, high levels of Kr-h1 in the PG inhibit ecdysteriod biosynthesis, thus maintaining juvenile status. JH biosynthesis is prevented by 20E in the corpus allatum, the other portion of the RG that produces JH, to ensure the occurrence of metamorphosis. Hence, antagonistic actions of JH and 20E within the RG determine developmental transitions in Drosophila. Our study proposes a mechanism of cross-talk between the two major hormones in the regulation of insect metamorphosis.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number1
DOIs
StatePublished - Jan 2 2018

Bibliographical note

Funding Information:
Drs. Michael O’Connor and Tetsu Shinoda for providing important reagents. This study was supported by the National Science Foundation of China (Grant 31620103917), the National Key Research and Development Program of China (Grant 2016YFD0101900), the Chinese Academy of Sciences (Grant 173176001000162007), and the National Science Foundation of China (Grants 31330072 and 31572325) (to S. Li).

Keywords

  • 20-hydroxyecdysone
  • Antagonistic action
  • Hormone biosynthesis
  • Juvenile hormone
  • Ring gland

ASJC Scopus subject areas

  • General

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