TY - JOUR
T1 - Anti-Müllerian hormone as an indicator of hemi-castrated unilateral cryptorchid horses
AU - Murase, Harutaka
AU - Saito, Shigeaki
AU - Amaya, Tomohiko
AU - Sato, Fumio
AU - Ball, Barry Allen
AU - Nambo, Yasuo
N1 - Publisher Copyright:
© 2015 Japanese Society of Equine Science.
PY - 2015/3/31
Y1 - 2015/3/31
N2 - Anti-Müllerian hormone (AMH), a glycoprotein secreted from the fetal testis, is responsible for regression of the Müllerian duct in the male fetus. The aim of this study was to evaluate the usefulness of serum AMH as a biomarker for diagnosis of cryptorchidism in horses. Serum AMH concentrations were measured in intact stallions, hemi-castrated unilateral cryptorchid stallions, and geldings. In addition, expression of AMH was characterized in cryptorchid testes by immunohistochemistry. Serum AMH was detected in intact stallions (n=11, 13.3 ± 1.8 ng/ml) and in hemi-castrated cryptorchid stallions (n=8, 17.6 ± 3.0 ng/ml), but not in geldings (n=6, all data were below the limit of detection). Immunolabeling for AMH was detected in Sertoli cells of undescended testes from cryptorchid horses as well as those of normal testes. Our findings indicate that the cryptorchid testis after hemicastration secretes AMH and that serum AMH concentrations may be a useful biomarker for diagnosis of equine cryptorchidism.
AB - Anti-Müllerian hormone (AMH), a glycoprotein secreted from the fetal testis, is responsible for regression of the Müllerian duct in the male fetus. The aim of this study was to evaluate the usefulness of serum AMH as a biomarker for diagnosis of cryptorchidism in horses. Serum AMH concentrations were measured in intact stallions, hemi-castrated unilateral cryptorchid stallions, and geldings. In addition, expression of AMH was characterized in cryptorchid testes by immunohistochemistry. Serum AMH was detected in intact stallions (n=11, 13.3 ± 1.8 ng/ml) and in hemi-castrated cryptorchid stallions (n=8, 17.6 ± 3.0 ng/ml), but not in geldings (n=6, all data were below the limit of detection). Immunolabeling for AMH was detected in Sertoli cells of undescended testes from cryptorchid horses as well as those of normal testes. Our findings indicate that the cryptorchid testis after hemicastration secretes AMH and that serum AMH concentrations may be a useful biomarker for diagnosis of equine cryptorchidism.
KW - Anti-Müllerian hormone (AMH)
KW - Cryptorchidism
KW - Equine
KW - Testis
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U2 - 10.1294/jes.26.15
DO - 10.1294/jes.26.15
M3 - Article
AN - SCOPUS:84934288798
SN - 1340-3516
VL - 26
SP - 15
EP - 20
JO - Journal of Equine Science
JF - Journal of Equine Science
IS - 1
ER -