Antifungal and antibacterial activities of indigenous Streptomyces isolates from saline farmlands: Prescreening, ribotyping and metabolic diversity

Imran Sajid, Clarisse Blandine Fotso Fondja Yao, Khaled Attia Shaaban, Shahida Hasnain, Hartmut Laatsch

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

A culture collection of 110 indigenous Streptomyces strains originally isolated from saline farmlands (Punjab, Pakistan) using stringent methods was screened biologically and chemically to investigate their potential for the production of bioactive secondary metabolites. In a biological screening the crude extracts obtained from the culture broth of selected strains were analysed for their activity against a set of test organisms, including Gram-positive, Gram-negative bacteria, fungi and microalgae using the disk diffusion bioassay method. Additionally a cytotoxicity test was performed by means of the brine shrimp microwell cytotoxicity assay. In a chemical screening each of the crude extracts was analysed by TLC using various staining reagents and by HPLC-MS/MS measurements. The results depicted an impressive chemical diversity of crude extracts produced by these strains. The taxonomic status of the selected strains was confirmed by preliminary physiological testing and 16S rRNA gene sequencing.

Original languageEnglish
Pages (from-to)601-610
Number of pages10
JournalWorld Journal of Microbiology and Biotechnology
Volume25
Issue number4
DOIs
StatePublished - Apr 2009

Bibliographical note

Funding Information:
Acknowledgments We are thankful to Dr. H. Frauendorf for mass spectra, and F. Lissy and A. Kohl for technical assistance. A financial support of this work by a grant from Higher Education Commission (HEC) of Pakistan under IRSIP is gratefully acknowledged.

Keywords

  • HPLC-MS/MS
  • Indigenous Streptomyces spp
  • Metabolite diversity
  • Prescreening
  • Ribotyping

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

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