A culture collection of 110 indigenous Streptomyces strains originally isolated from saline farmlands (Punjab, Pakistan) using stringent methods was screened biologically and chemically to investigate their potential for the production of bioactive secondary metabolites. In a biological screening the crude extracts obtained from the culture broth of selected strains were analysed for their activity against a set of test organisms, including Gram-positive, Gram-negative bacteria, fungi and microalgae using the disk diffusion bioassay method. Additionally a cytotoxicity test was performed by means of the brine shrimp microwell cytotoxicity assay. In a chemical screening each of the crude extracts was analysed by TLC using various staining reagents and by HPLC-MS/MS measurements. The results depicted an impressive chemical diversity of crude extracts produced by these strains. The taxonomic status of the selected strains was confirmed by preliminary physiological testing and 16S rRNA gene sequencing.
|Number of pages||10|
|Journal||World Journal of Microbiology and Biotechnology|
|State||Published - Apr 2009|
Bibliographical noteFunding Information:
Acknowledgments We are thankful to Dr. H. Frauendorf for mass spectra, and F. Lissy and A. Kohl for technical assistance. A financial support of this work by a grant from Higher Education Commission (HEC) of Pakistan under IRSIP is gratefully acknowledged.
- Indigenous Streptomyces spp
- Metabolite diversity
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology