Antioxidant and oxidative status in tissues of manganese superoxide dismutase transgenic mice

Wissam Ibrahim, Ung Soo Lee, Hsiu Chuan Yen, Davet K. St. Clair, Ching K. Chow

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Manganese superoxide dismutase (Mn-SOD) plays an important role in attenuating free radical-induced oxidative damage. The purpose of this research was to determine if increased expression of Mn-SOD gene alters intracellular redox status. Twelve week old male B6C3 mice, engineered to express human Mn-SOD in multiple organs, and their nontransgenic littermates were assessed for oxidative stress and antioxidant status in heart, brain, lung, skeletal muscle, liver, and kidney. Relative to their nontransgenic littermates, transgenic mice had significantly (p < .01) higher activity of Mn-SOD in heart, skeletal muscle, lung, and brain. Copper, zinc (Cu,Zn)-SOD activity was significantly higher in kidney, whereas catalase activity was lower in brain and liver. The activities of selenium (Se)-GSH peroxidase and non-Se-GSH peroxidase, and levels of vitamin E, ascorbic acid and GSH were not significantly different in any tissues measured between Mn-SOD transgenic mice and their nontransgenic controls. The levels of malondialdehyde were significantly lower in the muscle and heart of Mn-SOD mice, and conjugated dienes and protein carbonyls were not altered in any tissues measured. The results obtained showed that expression of human SOD gene did not systematical alter antioxidant systems or adversely affect the redox state of the transgenic mice. The results also suggest that expression of human SOD gene confers protection against peroxidative damage to membrane lipids. Copyright (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalFree Radical Biology and Medicine
Issue number3
StatePublished - Feb 1 2000

Bibliographical note

Funding Information:
Supported by Kentucky Agricultural Experiment station, and NIH grants.


  • Antioxidant status
  • Free radical
  • Mn-SOD
  • Oxidative stress
  • Transgenic mice

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)


Dive into the research topics of 'Antioxidant and oxidative status in tissues of manganese superoxide dismutase transgenic mice'. Together they form a unique fingerprint.

Cite this