Antioxidant peptides derived from hydrolysates of red tilapia (Oreochromis sp.) scale

Leidy Sierra, Hongbing Fan, José Zapata, Jianping Wu

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Processing red tilapia (Oreochromis sp.) yields a large quantity of scale, a byproduct of the aquaculture industry. This study focuses on evaluating the potential of red tilapia scales as a source of antioxidant peptides. Scales were ground and enzymatically hydrolyzed by Alcalase® 2.4 L. The hydrolysate obtained was fractionated to purify and identify the antioxidant peptides by membrane ultrafiltration and chromatographic technology guided by the antioxidant activity in rat vascular smooth muscle A7r5 cells. After ultrafiltration, the fraction with molecular weight of 3–10 kDa showed the highest activity and their chemical nature was hydrophilic. The sequence of twenty antioxidant peptides, containing 6–16 amino acids, was identified by liquid chromatography–tandem mass spectrometry. The results indicate that the red tilapia (Oreochromis sp.) scale could be a new natural source of antioxidant peptides.

Original languageEnglish
Article number111631
StatePublished - Jul 2021

Bibliographical note

Publisher Copyright:
© 2021 Elsevier Ltd


  • Antioxidant peptide
  • Byproduct
  • Fish scale
  • Oxidative stress
  • Purification and identification

ASJC Scopus subject areas

  • Food Science


Dive into the research topics of 'Antioxidant peptides derived from hydrolysates of red tilapia (Oreochromis sp.) scale'. Together they form a unique fingerprint.

Cite this