Antioxidant peptides derived from hydrolysates of red tilapia (Oreochromis sp.) scale

Leidy Sierra; Hongbing Fan; José Zapata; Jianping Wu

doi:10.1016/j.lwt.2021.111631

Antioxidant peptides derived from hydrolysates of red tilapia (Oreochromis sp.) scale

Leidy Sierra, Hongbing Fan, José Zapata, Jianping Wu

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Processing red tilapia (Oreochromis sp.) yields a large quantity of scale, a byproduct of the aquaculture industry. This study focuses on evaluating the potential of red tilapia scales as a source of antioxidant peptides. Scales were ground and enzymatically hydrolyzed by Alcalase® 2.4 L. The hydrolysate obtained was fractionated to purify and identify the antioxidant peptides by membrane ultrafiltration and chromatographic technology guided by the antioxidant activity in rat vascular smooth muscle A7r5 cells. After ultrafiltration, the fraction with molecular weight of 3–10 kDa showed the highest activity and their chemical nature was hydrophilic. The sequence of twenty antioxidant peptides, containing 6–16 amino acids, was identified by liquid chromatography–tandem mass spectrometry. The results indicate that the red tilapia (Oreochromis sp.) scale could be a new natural source of antioxidant peptides.

Original language	English
Article number	111631
Journal	LWT
Volume	146
DOIs	https://doi.org/10.1016/j.lwt.2021.111631
State	Published - Jul 2021

Bibliographical note

Funding Information:
The authors thank the Antioquia Department through “Sistema General de Regalías de Colombia” and Comité para el Desarrollo de la Investigación en la Universidad de Antioquia (CODI) through sustainability program, and Natural Sciences and Engineering Research Council of Canada (NSERC).

Funding Information:
The authors thank the Antioquia Department through ?Sistema General de Regal?as de Colombia? and Comit? para el Desarrollo de la Investigaci?n en la Universidad de Antioquia (CODI) through sustainability program, and Natural Sciences and Engineering Research Council of Canada (NSERC).

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

Antioxidant peptide
Byproduct
Fish scale
Oxidative stress
Purification and identification

ASJC Scopus subject areas

Food Science

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10.1016/j.lwt.2021.111631

Cite this

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title = "Antioxidant peptides derived from hydrolysates of red tilapia (Oreochromis sp.) scale",

abstract = "Processing red tilapia (Oreochromis sp.) yields a large quantity of scale, a byproduct of the aquaculture industry. This study focuses on evaluating the potential of red tilapia scales as a source of antioxidant peptides. Scales were ground and enzymatically hydrolyzed by Alcalase{\textregistered} 2.4 L. The hydrolysate obtained was fractionated to purify and identify the antioxidant peptides by membrane ultrafiltration and chromatographic technology guided by the antioxidant activity in rat vascular smooth muscle A7r5 cells. After ultrafiltration, the fraction with molecular weight of 3–10 kDa showed the highest activity and their chemical nature was hydrophilic. The sequence of twenty antioxidant peptides, containing 6–16 amino acids, was identified by liquid chromatography–tandem mass spectrometry. The results indicate that the red tilapia (Oreochromis sp.) scale could be a new natural source of antioxidant peptides.",

keywords = "Antioxidant peptide, Byproduct, Fish scale, Oxidative stress, Purification and identification",

author = "Leidy Sierra and Hongbing Fan and Jos{\'e} Zapata and Jianping Wu",

note = "Funding Information: The authors thank the Antioquia Department through “Sistema General de Regal{\'i}as de Colombia” and Comit{\'e} para el Desarrollo de la Investigaci{\'o}n en la Universidad de Antioquia (CODI) through sustainability program, and Natural Sciences and Engineering Research Council of Canada (NSERC). Funding Information: The authors thank the Antioquia Department through ?Sistema General de Regal?as de Colombia? and Comit? para el Desarrollo de la Investigaci?n en la Universidad de Antioquia (CODI) through sustainability program, and Natural Sciences and Engineering Research Council of Canada (NSERC). Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",

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doi = "10.1016/j.lwt.2021.111631",

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AU - Sierra, Leidy

AU - Fan, Hongbing

AU - Zapata, José

AU - Wu, Jianping

N1 - Funding Information: The authors thank the Antioquia Department through “Sistema General de Regalías de Colombia” and Comité para el Desarrollo de la Investigación en la Universidad de Antioquia (CODI) through sustainability program, and Natural Sciences and Engineering Research Council of Canada (NSERC). Funding Information: The authors thank the Antioquia Department through ?Sistema General de Regal?as de Colombia? and Comit? para el Desarrollo de la Investigaci?n en la Universidad de Antioquia (CODI) through sustainability program, and Natural Sciences and Engineering Research Council of Canada (NSERC). Publisher Copyright: © 2021 Elsevier Ltd

PY - 2021/7

Y1 - 2021/7

N2 - Processing red tilapia (Oreochromis sp.) yields a large quantity of scale, a byproduct of the aquaculture industry. This study focuses on evaluating the potential of red tilapia scales as a source of antioxidant peptides. Scales were ground and enzymatically hydrolyzed by Alcalase® 2.4 L. The hydrolysate obtained was fractionated to purify and identify the antioxidant peptides by membrane ultrafiltration and chromatographic technology guided by the antioxidant activity in rat vascular smooth muscle A7r5 cells. After ultrafiltration, the fraction with molecular weight of 3–10 kDa showed the highest activity and their chemical nature was hydrophilic. The sequence of twenty antioxidant peptides, containing 6–16 amino acids, was identified by liquid chromatography–tandem mass spectrometry. The results indicate that the red tilapia (Oreochromis sp.) scale could be a new natural source of antioxidant peptides.

AB - Processing red tilapia (Oreochromis sp.) yields a large quantity of scale, a byproduct of the aquaculture industry. This study focuses on evaluating the potential of red tilapia scales as a source of antioxidant peptides. Scales were ground and enzymatically hydrolyzed by Alcalase® 2.4 L. The hydrolysate obtained was fractionated to purify and identify the antioxidant peptides by membrane ultrafiltration and chromatographic technology guided by the antioxidant activity in rat vascular smooth muscle A7r5 cells. After ultrafiltration, the fraction with molecular weight of 3–10 kDa showed the highest activity and their chemical nature was hydrophilic. The sequence of twenty antioxidant peptides, containing 6–16 amino acids, was identified by liquid chromatography–tandem mass spectrometry. The results indicate that the red tilapia (Oreochromis sp.) scale could be a new natural source of antioxidant peptides.

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KW - Purification and identification

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Antioxidant peptides derived from hydrolysates of red tilapia (Oreochromis sp.) scale

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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