Abstract
Adriamycin (ADM) is a broad-spectrum antineoplastic antibiotic used to treat cancer patients. However, the usefulness of this drug is presently limited by the development of a dose-dependent cardiotoxicity. A current hypothesis for the ADM-induced cardiotoxicity is the production of reactive oxygen radicals by the drug. We utilized the fluorescent indicator 2',7'-dichlorodihydrofluorescein diacetate (DCFH/DA), in which fluorescence appears if reactive oxygen species (ROS) are present, to investigate the ability of ADM to generate reactive oxygen species and the potential protective effect of antioxidants in a cultured cardiomyocyte model. All three of the antioxidants (alpha-phenyl-tert-butyl nitrone (PBN), trolox, and 5-aminosalicyclic acid (5-ASA)) tested in our ADM-treated myocytes provided protection against the oxidative stress induced by the drug. These findings suggest that antioxidants modulate ADM-induced oxidative stress, and they are discussed in terms of a possible therapeutic strategy in the prevention of cardiotoxicity resulting from ADM administration. Copyright (C) 1999.
Original language | English |
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Pages (from-to) | 41-46 |
Number of pages | 6 |
Journal | Cancer Letters |
Volume | 136 |
Issue number | 1 |
DOIs | |
State | Published - Feb 8 1999 |
Bibliographical note
Funding Information:This work was supported in part by grants from the NIH and from Centaur Pharmaceuticals, Inc.
Keywords
- Adriamycin
- Antioxidants
- Cardiotoxicity
- Free radicals
ASJC Scopus subject areas
- Oncology
- Cancer Research