Antisense expression of hmg1 from Arabidopsis thaliana encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, reduces isoprenoid production in transgenic tobacco plants

G. C. Godoy-Hernandez, J. Chappell, T. P. Devarenne, E. Garcia-Pineda, A. A. Guevara-Garcia, E. Lozoya-Gloria

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

With the aim of studying the production of chloroplast isoprenoid derivatives, chimeric gene constructs comprising partial and full-length forms of the Arabidopsis thaliana hmg1 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), in sense and antisense forms were fused to a chloroplast transit peptide (CTP) sequence from a pea ribulose bisphosphate carboxylase/oxygenase (Rubisco) small subunit gene. These plasmids were placed downstream from the constitutive CaMV 35S promoter and introduced into tobacco (Nicotiana tabacum cv. Xanthi) by Agrobacterium-mediated transformation. Southern and northern blot analysis confirmed the transformation and transcription of the respective constructs within transgenic tobacco plants. In addition total and chloroplast specific HMGR enzyme activities, and the levels of chlorophyll, carotenoids and total sterols were analyzed. Results of these analyses suggest that neither partial nor full-length sense constructs had any effect on chloroplast isoprenoid production. However, the corresponding antisense constructs decreased general isoprenoid levels. The significance of these results is discussed.

Original languageEnglish
Pages (from-to)415-424
Number of pages10
JournalJournal of Plant Physiology
Volume153
Issue number3-4
DOIs
StatePublished - 1998

Bibliographical note

Funding Information:
We gratefully thank to Dr. Gerald Fink and Dr. Luis Herrera-Estrella for the kind gifts of plasmids, Ing. Beatriz Jimenez for sequencing analysis, BioI. Martha P. Chavez-Moctezuma for excellent technical assistance and Dr. Octavio Martinez de Ia Vega for statistic analysis. This work was supponed by a collaborative grant between the National Science Foundation (USA) and CONACYf (Mexico) N° N9212-1876. G.C.G.H. is recipient of a doctoral fellowship (N" 8399) from CONACYf (Mexico).

Keywords

  • Antisense
  • Arabidopsis thaliana
  • Chloroplast transit peptide
  • HMGR isoforms
  • Isoprenoids
  • Metabolons
  • Nicotiana tabacum cv. Xanthi

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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