Antisense inhibition of Na+/Ca2+ exchange during anoxia/reoxygenation in ventricular myocytes

B. N. Eigel, R. W. Hadley

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

This study investigated the role of the Na+/Ca2+ exchanger (NCX) in regulating cytosolic intracellular Ca2+ concentration ([Ca2+]i) during anoxia/reoxygenation in guinea pig ventricular myocytes. The hypothesis that the NCX is the predominant mechanism mediating [Ca2+]i overload in this model was tested through inhibition of NCX expression by an antisense oligonucleotide. Immunocytochemistry revealed that this antisense oligonucleotide, directed at the area around the start site of the guinea pig NCX1, specifically reduced NCX expression in cultured adult myocytes by 90 ± 4%. Antisense treatment inhibited evoked NCX activity by 94 ± 3% and decreased the rise in [Ca2+]i during anoxia/reoxygenation by 95 ± 3%. These data suggest that NCX is the predominant mechanism mediating Ca2+ overload during anoxia/reoxygenation in guinea-pig ventricular myocytes.

Original languageEnglish
Pages (from-to)H2184-H2190
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume281
Issue number5 50-5
DOIs
StatePublished - 2001

Keywords

  • Calcium
  • Heart
  • Hypoxia
  • Ischemia
  • Sodium/calcium exchanger

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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