Antitumour compounds from a saline soil isolate, Streptomyces griseoincarnatus CTF15

Imran Sajid, Khaled A. Shaaban, Shahida Hasnain

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


A new actinomycete strain designated as Streptomyces sp. CTF15 was isolated from a saline soil using casein-KNO3 agar medium. The strain Streptomyces sp. CTF15 exhibited promising antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Streptomyces viridochromogens Tu57 and high cytotoxicity (91.2% mortality) against Artimia salina in biological screening. The cultivation of this strain in a 50 L lab fermenter and subsequent isolation and purification by a series of chromatographic techniques and structure elucidation by MS and NMR analysis of the active metabolites revealed that it is a highly stable producer of resistomycin (1), tetracenomycin D (2) and actinomycin D (3), even under non-optimised culture conditions. The morphological, microscopic, biochemical and physiological characterisation suggested that the strain CTF15 belongs to the genus Streptomyces. A partial 16S rRNA gene sequence (1429 bp) from the strain CTF15 was determined and found to have high identity (99%) with Streptomyces griseoincarnatus. As such, this is the first report of a strain of S. griseoincarnatus capable of producing these three bioactive compounds simultaneously.

Original languageEnglish
Pages (from-to)549-559
Number of pages11
JournalNatural Product Research
Issue number5
StatePublished - Mar 2011

Bibliographical note

Funding Information:
We thank Prof. Dr Hartmut Laatsch, Institute of Organic and Biomolecular Chemistry, University of Gottingen, Germany, for providing, fermentation, MS and NMR analysis facilities. Financial support of this study by a grant from the Higher Education Commission (HEC) of Pakistan under IRSIP is gratefully acknowledged.


  • Streptomyces sp. CTF15
  • actinomycin D
  • resistomycin
  • tetracenomycin D

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry


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