APOΕ4 lowers energy expenditure in females and impairs glucose oxidation by increasing flux through aerobic glycolysis

Brandon C. Farmer, Holden C. Williams, Nicholas A. Devanney, Margaret A. Piron, Grant K. Nation, David J. Carter, Adeline E. Walsh, Rebika Khanal, Lyndsay E.A. Young, Jude C. Kluemper, Gabriela Hernandez, Elizabeth J. Allenger, Rachel Mooney, Lesley R. Golden, Cathryn T. Smith, J. Anthony Brandon, Vedant A. Gupta, Philip A. Kern, Matthew S. Gentry, Josh M. MorgantiRamon C. Sun, Lance A. Johnson

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Background: Cerebral glucose hypometabolism is consistently observed in individuals with Alzheimer’s disease (AD), as well as in young cognitively normal carriers of the Ε4 allele of Apolipoprotein E (APOE), the strongest genetic predictor of late-onset AD. While this clinical feature has been described for over two decades, the mechanism underlying these changes in cerebral glucose metabolism remains a critical knowledge gap in the field. Methods: Here, we undertook a multi-omic approach by combining single-cell RNA sequencing (scRNAseq) and stable isotope resolved metabolomics (SIRM) to define a metabolic rewiring across astrocytes, brain tissue, mice, and human subjects expressing APOE4. Results: Single-cell analysis of brain tissue from mice expressing human APOE revealed E4-associated decreases in genes related to oxidative phosphorylation, particularly in astrocytes. This shift was confirmed on a metabolic level with isotopic tracing of 13C-glucose in E4 mice and astrocytes, which showed decreased pyruvate entry into the TCA cycle and increased lactate synthesis. Metabolic phenotyping of E4 astrocytes showed elevated glycolytic activity, decreased oxygen consumption, blunted oxidative flexibility, and a lower rate of glucose oxidation in the presence of lactate. Together, these cellular findings suggest an E4-associated increase in aerobic glycolysis (i.e. the Warburg effect). To test whether this phenomenon translated to APOE4 humans, we analyzed the plasma metabolome of young and middle-aged human participants with and without the Ε4 allele, and used indirect calorimetry to measure whole body oxygen consumption and energy expenditure. In line with data from E4-expressing female mice, a subgroup analysis revealed that young female E4 carriers showed a striking decrease in energy expenditure compared to non-carriers. This decrease in energy expenditure was primarily driven by a lower rate of oxygen consumption, and was exaggerated following a dietary glucose challenge. Further, the stunted oxygen consumption was accompanied by markedly increased lactate in the plasma of E4 carriers, and a pathway analysis of the plasma metabolome suggested an increase in aerobic glycolysis. Conclusions: Together, these results suggest astrocyte, brain and system-level metabolic reprogramming in the presence of APOE4, a ‘Warburg like’ endophenotype that is observable in young females decades prior to clinically manifest AD.

Original languageEnglish
Article number62
JournalMolecular Neurodegeneration
Volume16
Issue number1
DOIs
StatePublished - Dec 2021

Bibliographical note

Funding Information:
This work was supported by the National Institute on Aging (BCF - F30AG06342201A; HCW- 1T32AG057461–01; JMM - 1RF1NS118558–01; LAJ - 1R01AG060056 and R01AG062550), the National Institute of General Medical Sciences (HCW- 5T32GM118292–03, LAJ- COBRE P20 GM103527, RCS - COBRE P20 GM121327), the National Institute of Neurological Disorders and Stroke (MSG - R01NS070899, R01NS070899-09S2, R35NS116824), the American Cancer Society Institutional Research Grant (RCS -#16–182-28), and the St Baldrick’s Foundation (Career Development Award to RCS). The project described was also supported by the National Center for Advancing Translational Sciences, National Institutes of Health (UL1TR001998) and the Redox Metabolism Shared Resource Facility of the University of Kentucky Markey Cancer Center (P30CA177558).

Funding Information:
The authors thank the Center for Clinical and Translational Science nursing staff for assistance with venipunctures, Dr. Arnold Stromberg, Dr. Richard Kryscio and Ning Li for statistics consultation, and Anna Wilwerding for her assistance in data input and organization. The authors would also like to thank Dr. Doug Harrison and Jim Begley, and are grateful for the support of the A&S Imaging Center provided by the College of Arts & Sciences, Department of Biology, University of Kentucky.

Publisher Copyright:
© 2021, The Author(s).

Keywords

  • APOE
  • Aerobic glycolysis
  • Alzheimer’s disease
  • Apolipoprotein E
  • Energy expenditure
  • Metabolism

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

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