APOE DNA methylation is altered in Lewy body dementia

Jessica Tulloch; Lesley Leong; Sunny Chen; C. Dirk Keene; Steven P. Millard; Andrew Shutes-David; Oscar L. Lopez; Julia Kofler; Jeffrey A. Kaye; Randy Woltjer; Peter T. Nelson; Janna H. Neltner; Gregory A. Jicha; Douglas Galasko; Eliezer Masliah; James B. Leverenz; Chang En Yu; Debby Tsuang

doi:10.1016/j.jalz.2018.02.005

APOE DNA methylation is altered in Lewy body dementia

Jessica Tulloch, Lesley Leong, Sunny Chen, C. Dirk Keene, Steven P. Millard, Andrew Shutes-David, Oscar L. Lopez, Julia Kofler, Jeffrey A. Kaye, Randy Woltjer, Peter T. Nelson, Janna H. Neltner, Gregory A. Jicha, Douglas Galasko, Eliezer Masliah, James B. Leverenz, Chang En Yu, Debby Tsuang

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Introduction: Inheritance of the ε4 allele of apolipoprotein E (APOE) increases a person's risk of developing both Alzheimer's disease (AD) and Lewy body dementia (LBD), yet the underlying mechanisms behind this risk are incompletely understood. The recent identification of reduced APOE DNA methylation in AD postmortem brains prompted this study to investigate APOE methylation in LBD. Methods: Genomic DNA from postmortem brain tissues (frontal lobe and cerebellum) of neuropathological pure (np) controls and npAD, LBD + AD, and npLBD subjects were bisulfite pyrosequenced. DNA methylation levels of two APOE subregions were then compared for these groups. Results: APOE DNA methylation was significantly reduced in npLBD compared with np controls, and methylation levels were lowest in the LBD + AD group. Discussion: Given that npLBD and npAD postmortem brains shared a similar reduction in APOE methylation, it is possible that an aberrant epigenetic change in APOE is linked to risk for both diseases.

Original language	English
Pages (from-to)	889-894
Number of pages	6
Journal	Alzheimer's and Dementia
Volume	14
Issue number	7
DOIs	https://doi.org/10.1016/j.jalz.2018.02.005
State	Published - Jul 2018

Bibliographical note

Publisher Copyright:
© 2018

Funding

Funding Sources: This work was supported in part by the U.S. Department of Veterans Affairs Office of Research and Development Biomedical Laboratory Research Program and by a grant from the National Institute of Neurological Disorders and Stroke (1R03NS103950). The contents do not represent the views of the U.S. Department of Veterans Affairs or the United States Government. Brain tissues were obtained from donors to the following research centers: University of Washington (UW) Neuropathology Core, which is supported by the Alzheimer's Disease Research Center (AG05136), and the NIA-supported Alzheimer's Disease Centers at the University of Pittsburgh (AG005133), University of California at San Diego (AG005131), University of Kentucky (AG028383), and Oregon Health and Science University (AG008017). Funding Sources: This work was supported in part by the U.S. Department of Veterans Affairs Office of Research and Development Biomedical Laboratory Research Program and by a grant from the National Institute of Neurological Disorders and Stroke (1R03NS103950). The contents do not represent the views of the U.S. Department of Veterans Affairs or the United States Government. Brain tissues were obtained from donors to the following research centers: University of Washington (UW) Neuropathology Core, which is supported by the Alzheimer's Disease Research Center ( AG05136 ), and the NIA-supported Alzheimer's Disease Centers at the University of Pittsburgh ( AG005133 ), University of California at San Diego ( AG005131 ), University of Kentucky ( AG028383 ), and Oregon Health and Science University ( AG008017 ).

Funders	Funder number
U.S. Department of Veterans Affairs Office of Research and Development Biomedical Laboratory Research Program
National Institute on Aging	P50AG005133
National Institute on Aging
Institute of Neurological Disorders and Stroke National Advisory Neurological Disorders and Stroke Council	1R03NS103950
Institute of Neurological Disorders and Stroke National Advisory Neurological Disorders and Stroke Council
Alzheimer's Disease Research Foundation
California Sea Grant, University of California, San Diego	AG005131
California Sea Grant, University of California, San Diego
Alzheimer's Disease Research Center, Emory University	AG05136
Alzheimer's Disease Research Center, Emory University
Oregon Health and Science University	AG008017
Oregon Health and Science University
University of Kentucky	AG028383
University of Kentucky
The George Washington University
University of California San Diego Health
University of Pittsburgh Medical Center, Children's Hospital of Pittsburgh	AG005133
University of Pittsburgh Medical Center, Children's Hospital of Pittsburgh

Keywords

Alzheimer's disease (AD)
Apolipoprotein E (APOE)
Cerebellum
DNA methylation
Dementia with Lewy bodies (DLB)
Differential methylation
Differentially methylated region (DMR)
Epigenetics
Frontal lobe
Human
Lewy body dementia (LBD)
Postmortem brain
Pyrosequencing

ASJC Scopus subject areas

Epidemiology
Health Policy
Developmental Neuroscience
Clinical Neurology
Geriatrics and Gerontology
Cellular and Molecular Neuroscience
Psychiatry and Mental health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jalz.2018.02.005

Cite this

Tulloch, J., Leong, L., Chen, S., Keene, C. D., Millard, S. P., Shutes-David, A., Lopez, O. L., Kofler, J., Kaye, J. A., Woltjer, R., Nelson, P. T., Neltner, J. H., Jicha, G. A., Galasko, D., Masliah, E., Leverenz, J. B., Yu, C. E., & Tsuang, D. (2018). APOE DNA methylation is altered in Lewy body dementia. Alzheimer's and Dementia, 14(7), 889-894. https://doi.org/10.1016/j.jalz.2018.02.005

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title = "APOE DNA methylation is altered in Lewy body dementia",

abstract = "Introduction: Inheritance of the ε4 allele of apolipoprotein E (APOE) increases a person's risk of developing both Alzheimer's disease (AD) and Lewy body dementia (LBD), yet the underlying mechanisms behind this risk are incompletely understood. The recent identification of reduced APOE DNA methylation in AD postmortem brains prompted this study to investigate APOE methylation in LBD. Methods: Genomic DNA from postmortem brain tissues (frontal lobe and cerebellum) of neuropathological pure (np) controls and npAD, LBD + AD, and npLBD subjects were bisulfite pyrosequenced. DNA methylation levels of two APOE subregions were then compared for these groups. Results: APOE DNA methylation was significantly reduced in npLBD compared with np controls, and methylation levels were lowest in the LBD + AD group. Discussion: Given that npLBD and npAD postmortem brains shared a similar reduction in APOE methylation, it is possible that an aberrant epigenetic change in APOE is linked to risk for both diseases.",

keywords = "Alzheimer's disease (AD), Apolipoprotein E (APOE), Cerebellum, DNA methylation, Dementia with Lewy bodies (DLB), Differential methylation, Differentially methylated region (DMR), Epigenetics, Frontal lobe, Human, Lewy body dementia (LBD), Postmortem brain, Pyrosequencing",

author = "Jessica Tulloch and Lesley Leong and Sunny Chen and Keene, \{C. Dirk\} and Millard, \{Steven P.\} and Andrew Shutes-David and Lopez, \{Oscar L.\} and Julia Kofler and Kaye, \{Jeffrey A.\} and Randy Woltjer and Nelson, \{Peter T.\} and Neltner, \{Janna H.\} and Jicha, \{Gregory A.\} and Douglas Galasko and Eliezer Masliah and Leverenz, \{James B.\} and Yu, \{Chang En\} and Debby Tsuang",

note = "Publisher Copyright: {\textcopyright} 2018",

year = "2018",

month = jul,

doi = "10.1016/j.jalz.2018.02.005",

language = "English",

volume = "14",

pages = "889--894",

number = "7",

}

TY - JOUR

T1 - APOE DNA methylation is altered in Lewy body dementia

AU - Tulloch, Jessica

AU - Leong, Lesley

AU - Chen, Sunny

AU - Keene, C. Dirk

AU - Millard, Steven P.

AU - Shutes-David, Andrew

AU - Lopez, Oscar L.

AU - Kofler, Julia

AU - Kaye, Jeffrey A.

AU - Woltjer, Randy

AU - Nelson, Peter T.

AU - Neltner, Janna H.

AU - Jicha, Gregory A.

AU - Galasko, Douglas

AU - Masliah, Eliezer

AU - Leverenz, James B.

AU - Yu, Chang En

AU - Tsuang, Debby

PY - 2018/7

Y1 - 2018/7

N2 - Introduction: Inheritance of the ε4 allele of apolipoprotein E (APOE) increases a person's risk of developing both Alzheimer's disease (AD) and Lewy body dementia (LBD), yet the underlying mechanisms behind this risk are incompletely understood. The recent identification of reduced APOE DNA methylation in AD postmortem brains prompted this study to investigate APOE methylation in LBD. Methods: Genomic DNA from postmortem brain tissues (frontal lobe and cerebellum) of neuropathological pure (np) controls and npAD, LBD + AD, and npLBD subjects were bisulfite pyrosequenced. DNA methylation levels of two APOE subregions were then compared for these groups. Results: APOE DNA methylation was significantly reduced in npLBD compared with np controls, and methylation levels were lowest in the LBD + AD group. Discussion: Given that npLBD and npAD postmortem brains shared a similar reduction in APOE methylation, it is possible that an aberrant epigenetic change in APOE is linked to risk for both diseases.

AB - Introduction: Inheritance of the ε4 allele of apolipoprotein E (APOE) increases a person's risk of developing both Alzheimer's disease (AD) and Lewy body dementia (LBD), yet the underlying mechanisms behind this risk are incompletely understood. The recent identification of reduced APOE DNA methylation in AD postmortem brains prompted this study to investigate APOE methylation in LBD. Methods: Genomic DNA from postmortem brain tissues (frontal lobe and cerebellum) of neuropathological pure (np) controls and npAD, LBD + AD, and npLBD subjects were bisulfite pyrosequenced. DNA methylation levels of two APOE subregions were then compared for these groups. Results: APOE DNA methylation was significantly reduced in npLBD compared with np controls, and methylation levels were lowest in the LBD + AD group. Discussion: Given that npLBD and npAD postmortem brains shared a similar reduction in APOE methylation, it is possible that an aberrant epigenetic change in APOE is linked to risk for both diseases.

KW - Alzheimer's disease (AD)

KW - Apolipoprotein E (APOE)

KW - Cerebellum

KW - DNA methylation

KW - Dementia with Lewy bodies (DLB)

KW - Differential methylation

KW - Differentially methylated region (DMR)

KW - Epigenetics

KW - Frontal lobe

KW - Human

KW - Lewy body dementia (LBD)

KW - Postmortem brain

KW - Pyrosequencing

UR - https://www.scopus.com/pages/publications/85044739296

UR - https://www.scopus.com/inward/citedby.url?scp=85044739296&partnerID=8YFLogxK

U2 - 10.1016/j.jalz.2018.02.005

DO - 10.1016/j.jalz.2018.02.005

M3 - Article

C2 - 29544979

AN - SCOPUS:85044739296

SN - 1552-5260

VL - 14

SP - 889

EP - 894

JO - Alzheimer's and Dementia

JF - Alzheimer's and Dementia

IS - 7

ER -

APOE DNA methylation is altered in Lewy body dementia

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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