Apolipoprotein concentrations are elevated in malignant ovarian cyst fluids suggesting that lipoprotein metabolism is dysregulated in epithelial ovarian cancer

Iwona Podzielinski, Brook A. Saunders, Kimberly D. Kimbler, Adam J. Branscum, Eric T. Fung, Paul D. Depriest, John R. Van Nagell, Frederick R. Ueland, Andre T. Baron

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

SELDI-TOF MS analysis of ovarian cyst fluids revealed that peaks m/z 8696 and 8825 discriminate malignant, borderline, and benign tumors. These peaks correspond to isoforms of apoA2. ELISA demonstrates that apoA1, A2, B, C2, C3, and E cyst fluid concentrations are uncorrelated and higher in malignant ovarian tumors, but only apoA2, apoE, and age are independent classifiers of malignant ovarian tumors, yielding 55.1% sensitivity, 95% specificity, and 88.1% accuracy to discern malignant from benign and borderline tumors. These data suggest that lipoprotein metabolism is dysregulated in ovarian cancer and that apoA2 and apoE warrant further investigation as ovarian tumor biomarkers.

Original languageEnglish
Pages (from-to)258-272
Number of pages15
JournalCancer Investigation
Volume31
Issue number4
DOIs
StatePublished - May 2013

Bibliographical note

Funding Information:
This research was supported by development funds from the Division of Gynecologic Oncology, University of Kentucky; University of Kentucky, General Clinical Research Center (M01-RR02602) for apolipoprotein concentration measurements using LINCOplex ELISA kits and Luminex©R100 instrument; Ladies for Life Foundation; and Vermillion, Inc., which provided technical support, ProteinChip©R arrays and reagents, and access to a ProteinChip©R Reader Series 4000 mass spectrometer through a research agreement with the University of Kentucky.

Funding

This research was supported by development funds from the Division of Gynecologic Oncology, University of Kentucky; University of Kentucky, General Clinical Research Center (M01-RR02602) for apolipoprotein concentration measurements using LINCOplex ELISA kits and Luminex©R100 instrument; Ladies for Life Foundation; and Vermillion, Inc., which provided technical support, ProteinChip©R arrays and reagents, and access to a ProteinChip©R Reader Series 4000 mass spectrometer through a research agreement with the University of Kentucky.

FundersFunder number
Division of Gynecologic Oncology, University of Kentucky
University of Kentucky General Clinical Research CenterM01-RR02602
Ladies for Life Foundation
Vermillion Inc.
National Center for Research ResourcesM01RR002602
University of Kentucky

    Keywords

    • Cancer biomarkers
    • Detection/diagnosis
    • Ovarian cancer
    • Protein profiling
    • Tumor biology

    ASJC Scopus subject areas

    • Oncology
    • Cancer Research

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