Apolipoprotein concentrations are elevated in malignant ovarian cyst fluids suggesting that lipoprotein metabolism is dysregulated in epithelial ovarian cancer

Iwona Podzielinski; Brook A. Saunders; Kimberly D. Kimbler; Adam J. Branscum; Eric T. Fung; Paul D. Depriest; John R. Van Nagell; Frederick R. Ueland; Andre T. Baron

doi:10.3109/07357907.2013.789896

Apolipoprotein concentrations are elevated in malignant ovarian cyst fluids suggesting that lipoprotein metabolism is dysregulated in epithelial ovarian cancer

Iwona Podzielinski, Brook A. Saunders, Kimberly D. Kimbler, Adam J. Branscum, Eric T. Fung, Paul D. Depriest, John R. Van Nagell, Frederick R. Ueland, Andre T. Baron

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

SELDI-TOF MS analysis of ovarian cyst fluids revealed that peaks m/z 8696 and 8825 discriminate malignant, borderline, and benign tumors. These peaks correspond to isoforms of apoA2. ELISA demonstrates that apoA1, A2, B, C2, C3, and E cyst fluid concentrations are uncorrelated and higher in malignant ovarian tumors, but only apoA2, apoE, and age are independent classifiers of malignant ovarian tumors, yielding 55.1% sensitivity, 95% specificity, and 88.1% accuracy to discern malignant from benign and borderline tumors. These data suggest that lipoprotein metabolism is dysregulated in ovarian cancer and that apoA2 and apoE warrant further investigation as ovarian tumor biomarkers.

Original language	English
Pages (from-to)	258-272
Number of pages	15
Journal	Cancer Investigation
Volume	31
Issue number	4
DOIs	https://doi.org/10.3109/07357907.2013.789896
State	Published - May 2013

Bibliographical note

Funding Information:
This research was supported by development funds from the Division of Gynecologic Oncology, University of Kentucky; University of Kentucky, General Clinical Research Center (M01-RR02602) for apolipoprotein concentration measurements using LINCOplex ELISA kits and Luminex©R100 instrument; Ladies for Life Foundation; and Vermillion, Inc., which provided technical support, ProteinChip©R arrays and reagents, and access to a ProteinChip©R Reader Series 4000 mass spectrometer through a research agreement with the University of Kentucky.

Keywords

Cancer biomarkers
Detection/diagnosis
Ovarian cancer
Protein profiling
Tumor biology

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/07357907.2013.789896

Cite this

Podzielinski, I., Saunders, B. A., Kimbler, K. D., Branscum, A. J., Fung, E. T., Depriest, P. D., Van Nagell, J. R., Ueland, F. R., & Baron, A. T. (2013). Apolipoprotein concentrations are elevated in malignant ovarian cyst fluids suggesting that lipoprotein metabolism is dysregulated in epithelial ovarian cancer. Cancer Investigation, 31(4), 258-272. https://doi.org/10.3109/07357907.2013.789896

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title = "Apolipoprotein concentrations are elevated in malignant ovarian cyst fluids suggesting that lipoprotein metabolism is dysregulated in epithelial ovarian cancer",

abstract = "SELDI-TOF MS analysis of ovarian cyst fluids revealed that peaks m/z 8696 and 8825 discriminate malignant, borderline, and benign tumors. These peaks correspond to isoforms of apoA2. ELISA demonstrates that apoA1, A2, B, C2, C3, and E cyst fluid concentrations are uncorrelated and higher in malignant ovarian tumors, but only apoA2, apoE, and age are independent classifiers of malignant ovarian tumors, yielding 55.1% sensitivity, 95% specificity, and 88.1% accuracy to discern malignant from benign and borderline tumors. These data suggest that lipoprotein metabolism is dysregulated in ovarian cancer and that apoA2 and apoE warrant further investigation as ovarian tumor biomarkers.",

keywords = "Cancer biomarkers, Detection/diagnosis, Ovarian cancer, Protein profiling, Tumor biology",

author = "Iwona Podzielinski and Saunders, {Brook A.} and Kimbler, {Kimberly D.} and Branscum, {Adam J.} and Fung, {Eric T.} and Depriest, {Paul D.} and {Van Nagell}, {John R.} and Ueland, {Frederick R.} and Baron, {Andre T.}",

note = "Funding Information: This research was supported by development funds from the Division of Gynecologic Oncology, University of Kentucky; University of Kentucky, General Clinical Research Center (M01-RR02602) for apolipoprotein concentration measurements using LINCOplex ELISA kits and Luminex{\textcopyright}R100 instrument; Ladies for Life Foundation; and Vermillion, Inc., which provided technical support, ProteinChip{\textcopyright}R arrays and reagents, and access to a ProteinChip{\textcopyright}R Reader Series 4000 mass spectrometer through a research agreement with the University of Kentucky.",

year = "2013",

month = may,

doi = "10.3109/07357907.2013.789896",

language = "English",

volume = "31",

pages = "258--272",

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}

Podzielinski, I, Saunders, BA, Kimbler, KD, Branscum, AJ, Fung, ET, Depriest, PD, Van Nagell, JR, Ueland, FR & Baron, AT 2013, 'Apolipoprotein concentrations are elevated in malignant ovarian cyst fluids suggesting that lipoprotein metabolism is dysregulated in epithelial ovarian cancer', Cancer Investigation, vol. 31, no. 4, pp. 258-272. https://doi.org/10.3109/07357907.2013.789896

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T1 - Apolipoprotein concentrations are elevated in malignant ovarian cyst fluids suggesting that lipoprotein metabolism is dysregulated in epithelial ovarian cancer

AU - Podzielinski, Iwona

AU - Saunders, Brook A.

AU - Kimbler, Kimberly D.

AU - Branscum, Adam J.

AU - Fung, Eric T.

AU - Depriest, Paul D.

AU - Van Nagell, John R.

AU - Ueland, Frederick R.

AU - Baron, Andre T.

N1 - Funding Information: This research was supported by development funds from the Division of Gynecologic Oncology, University of Kentucky; University of Kentucky, General Clinical Research Center (M01-RR02602) for apolipoprotein concentration measurements using LINCOplex ELISA kits and Luminex©R100 instrument; Ladies for Life Foundation; and Vermillion, Inc., which provided technical support, ProteinChip©R arrays and reagents, and access to a ProteinChip©R Reader Series 4000 mass spectrometer through a research agreement with the University of Kentucky.

PY - 2013/5

Y1 - 2013/5

N2 - SELDI-TOF MS analysis of ovarian cyst fluids revealed that peaks m/z 8696 and 8825 discriminate malignant, borderline, and benign tumors. These peaks correspond to isoforms of apoA2. ELISA demonstrates that apoA1, A2, B, C2, C3, and E cyst fluid concentrations are uncorrelated and higher in malignant ovarian tumors, but only apoA2, apoE, and age are independent classifiers of malignant ovarian tumors, yielding 55.1% sensitivity, 95% specificity, and 88.1% accuracy to discern malignant from benign and borderline tumors. These data suggest that lipoprotein metabolism is dysregulated in ovarian cancer and that apoA2 and apoE warrant further investigation as ovarian tumor biomarkers.

AB - SELDI-TOF MS analysis of ovarian cyst fluids revealed that peaks m/z 8696 and 8825 discriminate malignant, borderline, and benign tumors. These peaks correspond to isoforms of apoA2. ELISA demonstrates that apoA1, A2, B, C2, C3, and E cyst fluid concentrations are uncorrelated and higher in malignant ovarian tumors, but only apoA2, apoE, and age are independent classifiers of malignant ovarian tumors, yielding 55.1% sensitivity, 95% specificity, and 88.1% accuracy to discern malignant from benign and borderline tumors. These data suggest that lipoprotein metabolism is dysregulated in ovarian cancer and that apoA2 and apoE warrant further investigation as ovarian tumor biomarkers.

KW - Cancer biomarkers

KW - Detection/diagnosis

KW - Ovarian cancer

KW - Protein profiling

KW - Tumor biology

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Apolipoprotein concentrations are elevated in malignant ovarian cyst fluids suggesting that lipoprotein metabolism is dysregulated in epithelial ovarian cancer

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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