Abstract
SELDI-TOF MS analysis of ovarian cyst fluids revealed that peaks m/z 8696 and 8825 discriminate malignant, borderline, and benign tumors. These peaks correspond to isoforms of apoA2. ELISA demonstrates that apoA1, A2, B, C2, C3, and E cyst fluid concentrations are uncorrelated and higher in malignant ovarian tumors, but only apoA2, apoE, and age are independent classifiers of malignant ovarian tumors, yielding 55.1% sensitivity, 95% specificity, and 88.1% accuracy to discern malignant from benign and borderline tumors. These data suggest that lipoprotein metabolism is dysregulated in ovarian cancer and that apoA2 and apoE warrant further investigation as ovarian tumor biomarkers.
Original language | English |
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Pages (from-to) | 258-272 |
Number of pages | 15 |
Journal | Cancer Investigation |
Volume | 31 |
Issue number | 4 |
DOIs | |
State | Published - May 2013 |
Bibliographical note
Funding Information:This research was supported by development funds from the Division of Gynecologic Oncology, University of Kentucky; University of Kentucky, General Clinical Research Center (M01-RR02602) for apolipoprotein concentration measurements using LINCOplex ELISA kits and Luminex©R100 instrument; Ladies for Life Foundation; and Vermillion, Inc., which provided technical support, ProteinChip©R arrays and reagents, and access to a ProteinChip©R Reader Series 4000 mass spectrometer through a research agreement with the University of Kentucky.
Funding
This research was supported by development funds from the Division of Gynecologic Oncology, University of Kentucky; University of Kentucky, General Clinical Research Center (M01-RR02602) for apolipoprotein concentration measurements using LINCOplex ELISA kits and Luminex©R100 instrument; Ladies for Life Foundation; and Vermillion, Inc., which provided technical support, ProteinChip©R arrays and reagents, and access to a ProteinChip©R Reader Series 4000 mass spectrometer through a research agreement with the University of Kentucky.
Funders | Funder number |
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Division of Gynecologic Oncology, University of Kentucky | |
University of Kentucky General Clinical Research Center | M01-RR02602 |
Ladies for Life Foundation | |
Vermillion Inc. | |
National Center for Research Resources | M01RR002602 |
University of Kentucky |
Keywords
- Cancer biomarkers
- Detection/diagnosis
- Ovarian cancer
- Protein profiling
- Tumor biology
ASJC Scopus subject areas
- Oncology
- Cancer Research